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Introduction: To investigate adrenal tumors of patients operated on at the University Hospital Center Osijek from 2016 to 2019 for the purpose of examining the location and histopathological findings of the tumors and determining whether there is a difference related to that in terms of the age and sex of the subjects. Subjects and Methods: This was a cross-sectional study with historical data analysis. The subjects were patients of both sexes (N=23) diagnosed with adrenal tumors (N=23) who underwent surgery at the University Hospital Center Osijek in the period from 2016 to 2019. The archive was used in the University Hospital Center Osijek. Results: Adrenal tumors were more often unilateral than bilateral. No differences were found in the localization of tumors of the right and left adrenal glands. Benign tumors were more common than malignant tumors and were the most common adenoma. Seventeen women underwent surgery and were 10 years older on average (61.6 years). There were no significant differences regarding tumor occurrence with respect to the patients’ age. Benign tumors were more common in women, but the difference was not statistically significant. Regarding histopathological findings, women were more likely to have adenoma (N=10) and hyperplasia (N=4), while men had other benign tumors (N=3) and metastatic tumors (N=1). However, the difference was not statistically significant. Conclusion: In patients who have undergone surgery for adrenal tumors, there were more unilateral tumors compared to bilateral ones, but there was no difference in tumor localization (left and right). Benign tumors were more common, the most common of which were adenomas. There were no significant differences with respect to age and sex. (Lekić I, Banović V, Marjanović K, Kovačić B, Feldi I, Bačun T. Pathohistological Diagnosis of Adrenal Tumors: Experience of a Single Center. SEEMEDJ 2020; 4(2); 108-112)