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Cyanobacteria are known for forming associations with various animals, including sea turtles, yet our understanding of cyanobacteria associated with sea turtles remains limited. This study aims to address this knowledge gap by investigating the diversity of cyanobacteria in biofilm samples from loggerhead sea turtle carapaces, utilizing a 16S rRNA gene amplicon sequencing approach. The predominant cyanobacterial order identified was Nodosilineales, with the genus Rhodoploca having the highest relative abundance. Our results suggest that cyanobacterial communities become more diverse as sea turtles age, as we observed a positive correlation between community diversity and the length of a sea turtle's carapace. Since larger and older turtles predominantly utilize neritic habitats, the shift to a more diverse cyanobacterial community aligned with a change in loggerhead habitat. Our research provides detailed insights into the cyanobacterial communities associated with loggerhead sea turtles, establishing a foundation for future studies delving into this fascinating ecological relationship and its potential implications for sea turtle conservation. [ABSTRACT FROM AUTHOR] more...

Simple Summary: Loggerhead sea turtles have a flexible distribution from oceanic-pelagic to neritic-benthic areas. Their dietary preferences are expected to change from pelagic prey to benthic prey along with habitat use while they increase in size. This study evaluated the gastrointestinal contents of 150 loggerhead sea turtles stranded and/or bycaught along the Italian coasts of the central Adriatic Sea and central Tyrrhenian sea to assess the most common prey phyla. Information on the ingestion of litter has been collected for each carcass as well. This work highlights the need to deepen the knowledge of the feeding ecology and distribution of sea turtles at any developmental stage. According to their life stage, the loggerhead sea turtle (Caretta caretta) is found in a wide range of habitats, from neritic to more oceanic areas. Their feeding habits are expected to change as they develop, along with habitat use. Juvenile sea turtles are hypothesized to feed on pelagic species in oceanic areas, shifting to more benthic prey during the subadult and adult stages. We analyzed the gastrointestinal content from 150 loggerhead sea turtles stranded and/or bycaught along the Adriatic coast of the Abruzzo and Molise regions (n = 89) and the Tyrrhenian coast of the Lazio and Campania regions (n = 61) from 2018 to 2021. Food items were identified to the lowest taxonomic level, and the frequency of occurrence was calculated for each taxon and most recurrent species to assess changes in prey selection during the development. The marine litter was categorized, and the frequency of occurrence was calculated for the ingestion of litter. The most recurrent taxonomic prey group recorded in the Adriatic sample was Arthropoda (94%), followed by Mollusca (63%) and Chordata (34%). In the Tyrrhenian sample, loggerhead sea turtles fed mostly on Mollusca (84%), Arthropoda (38%), and Chordata (26%). Surprisingly, the Adriatic-Tyrrhenian sample groups showed similar feeding behavior between juveniles, subadults, and adults. A similar correlation has been observed concerning the ingestion of litter. Moreover, this study confirms the opportunistic feeding behavior of loggerhead sea turtles and their high adaptability. [ABSTRACT FROM AUTHOR] more...

Abstract There is evidence that polycyclic aromatic hydrocarbons (PAHs) are consistently the predominant organic contaminants in concentration found in loggerhead sea turtles (Caretta caretta) from the North and Central Adriatic Sea. Hence this study investigates the PAH toxicity to loggerheads by using a particular set of genes [ i.e. CYP1B, CAT, GPX, GSTT1, SOD3, DNMT1, Epoxide hydrolase 1 (EPHX1), Poly (ADP-ribose) polymerase 1 (PARP1), Lamin-A/C isoform 3 (LMNA), Talin 1 (TLN1), Annexin A1 (ANXA1)] whose altered expression is potentially dependent on and specific for the PAH-related mechanism of action. Twenty healthy juvenile loggerheads were thus divided into high and low exposure groups (mean of ΣPAHs: 80.34 ng mL−1 vs. 8.84 ng mL−1, P < 0.0001) according to the median split of ΣPAHs. Interestingly, we found that the whole blood mRNA levels of each gene biomarker tested were significantly increased in high PAH-exposed turtles thus proving to be useful for the biological monitoring of PAH toxicity and hematotoxicity in sea turtles. Graphical abstract Image 1 Highlights • We examined potential PAH-related biomarkers in whole blood of loggerheads captured alive. • The PAH levels can be associated with selected gene biomarkers transcript levels. • CYP1B, NADP, LMNA and DNMT1 showed the highest gene expression levels. • Whole blood TBARS have been shown to be elevated in high PAH-exposed turtles. • We suggest links between PAH exposure and potential adverse outcomes in sea turtles. This study provides new insights into the relationship between changes in mRNA abundance profiles of specific gene biomarkers and plasma levels of total PAHs in C. caretta turtles. Our results further support the potential of using whole blood cell gene biomarkers for monitoring the impact of environmental contaminants on sea turtles. [ABSTRACT FROM AUTHOR] more...

The present study sought to determine bioaccumulation and potential molecular effects of four of the most environmentally significant sunscreen agents in juvenile loggerhead sea turtles (Caretta caretta) from the Italian coasts of the central Adriatic Sea. Each of these sunscreen agents were found in most of the plasma samples analyzed, with benzophenone-3 as the dominant species. Total concentrations of the sunscreen agents ranged from not detected (−1. Almost all of the gene biomarkers of inflammation, oxidative stress and hormonal activity assessed by RT-qPCR in blood tissue showed significant positive correlations with total sunscreen agent concentrations. The present study is the first to provide data on contamination by sunscreen agents in loggerheads, and indicates that these chemicals tend to bioaccumulate and exert potential adverse effects in sea turtles. • Environmentally relevant sunscreen agents were detected in Adriatic loggerheads. • Total concentrations of the UV filters ranged from not detected to 28.43 g mL−1. • Blood gene expression biomarkers were positively associated with total UV filters. [ABSTRACT FROM AUTHOR] more...