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2 results on '"Ceren Turkcan Kayhan"'

1. DNA isolation by galactoacrylate-based nano-poly(HEMA-co-Gal-OPA) nanopolymers

Author

Sinan Akgöl, Deniz Aktaş Uygun, Yeşim Salman, Murat Uygun, Meryem Koruyucu, Fulden Zeynep Ural, Ceren Turkcan Kayhan, and Adil Denizli

Subjects
Polymers, Surface Properties, Biomedical Engineering, Biophysics, Bioengineering, 02 engineering and technology, Biology, 010402 general chemistry, 01 natural sciences, law.invention, Biomaterials, chemistry.chemical_compound, law, Nano, Temperature, Water, DNA, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, DNA extraction, Molecular biology, 0104 chemical sciences, Nanostructures, chemistry, Recombinant DNA, Adsorption, 0210 nano-technology
Abstract

Isolation of DNA is one of the important processes for biotechnological applications such as investigation of DNA structures and functions, recombinant DNA preparations, identification of genetic factors and diagnosis and treatment of genetic disorders. The aim of this study was to synthesis and characterizes the galactoacrylate based nanopolymers with high surface area and to investigate the usability of these synthesized nanopolymers for DNA isolation studies. Nanopolymers were synthesized by the surfactant free emulsion polymerization technique by using the monomers of 2-hydroxyl ethylmethacrylate and 6-O-(2

Published
2017

2. Synthesis and characterization of cryogel structures for isolation of EPSs from Botryococcus braunii

Author

Irem Cemre Turu, Aslihan Kazan, Ceren Turkcan-Kayhan, Ece Yildiz-Ozturk, Sinan Akgöl, and Ozlem Yesil-Celiktas

Subjects
Polymers and Plastics, Surface Properties, 02 engineering and technology, Chemistry Techniques, Synthetic, Methacrylate, 01 natural sciences, law.invention, Polymerization, Adsorption, Magazine, law, Chlorophyta, Polysaccharides, Materials Chemistry, medicine, Botryococcus braunii, Concanavalin A, Fourier transform infrared spectroscopy, Chromatography, Statistical design, biology, 010405 organic chemistry, Chemistry, Organic Chemistry, Temperature, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, biology.protein, Methacrylates, Swelling, medicine.symptom, 0210 nano-technology, Hydrophobic and Hydrophilic Interactions, Cryogels
Abstract

In this study, the objective was to separate exopolysaccharides (EPSs) released in the broth subsequent to outdoor cultivation of Botryococcus braunii. For this, poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were synthesized. After that, the surface was modified by coupling Concanavalin A. Box-Behnken statistical design was used to evaluate the effect of freezing temperature, Con A concentration and flow rate on Con A binding capacity. Optimum synthesis conditions were elicited as -14.48°C freezing temperature, 1.00mg/ml Con A concentration and 0.30ml/min flow rate yielding 3.18mg Con A/g cryogel, whereas -16°C, 1.00mg/ml and 0.30ml/min yielded the highest (3.38mg) binding capacity in experimental cryogel preparation. The EPS adsorption capacity of the optimum cryogel column was found as 3.26mg EPS/g cryogel corresponding to adsorption yield of 80%. Besides; swelling test, elemental analysis, Micro-CT, SEM and FTIR analysis were carried out for characterization of the synthesized cryogels.

Published
2016

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