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1. Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted in an Italian Reference Hospital

Author

Annalisa Mondi, Patrizia Lorenzini, Concetta Castilletti, Roberta Gagliardini, Eleonora Lalle, Angela Corpolongo, Maria Beatrice Valli, Fabrizio Taglietti, Stefania Cicalini, Laura Loiacono, Francesco Di Gennaro, Gianpiero D’Offizi, Fabrizio Palmieri, Emanuele Nicastri, Chiara Agrati, Nicola Petrosillo, Giuseppe Ippolito, Francesco Vaia, Enrico Girardi, Maria Rosaria Capobianchi, Andrea Antinori, Sara Zito, Maria Alessandra Abbonizio, Amina Abdeddaim, Elisabetta Agostini, Fabrizio Albarello, Gioia Amadei, Alessandra Amendola, Maria Assunta Antonica, Mario Antonini, Tommaso Ascoli Bartoli, Francesco Baldini, Raffaella Barbaro, Barbara Bartolini, Rita Bellagamba, Martina Benigni, Nazario Bevilacqua, Gianluigi Biava, Michele Bibas, Licia Bordi, Veronica Bordoni, Evangelo Boumis, Marta Branca, Rosanna Buonomo, Donatella Busso, Marta Camici, Paolo Campioni, Flaminia Canichella, Alessandro Capone, Cinzia Caporale, Emanuela Caraffa, Ilaria Caravella, Fabrizio Carletti, Adriana Cataldo, Stefano Cerilli, Carlotta Cerva, Roberta Chiappini, Pierangelo Chinello, Maria Assunta Cianfarani, Carmine Ciaralli, Claudia Cimaglia, Nicola Cinicola, Veronica Ciotti, Francesca Colavita, Massimo Cristofaro, Salvatore Curiale, Alessandra D’Abramo, Cristina Dantimi, Alessia De Angelis, Giada De Angelis, Maria Grazia De Palo, Federico De Zottis, Virginia Di Bari, Rachele Di Lorenzo, Federica Di Stefano, Davide Donno, Francesca Evangelista, Francesca Faraglia, Anna Farina, Federica Ferraro, Lorena Fiorentini, Andrea Frustaci, Matteo Fusetti, Vincenzo Galati, Paola Gallì, Gabriele Garotto, Ilaria Gaviano, Saba Gebremeskel Tekle, Maria Letizia Giancola, Filippo Giansante, Emanuela Giombini, Guido Granata, Maria Cristina Greci, Elisabetta Grilli, Susanna Grisetti, Gina Gualano, Fabio Iacomi, Marta Iaconi, Giuseppina Iannicelli, Carlo Inversi, Maria Elena Lamanna, Simone Lanini, Daniele Lapa, Luciana Lepore, Raffaella Libertone, Raffaella Lionetti, Giuseppina Liuzzi, Andrea Lucia, Franco Lufrani, Manuela Macchione, Gaetano Maffongelli, Alessandra Marani, Luisa Marchioni, Andrea Mariano, Maria Cristina Marini, Micaela Maritti, Annelisa Mastrobattista, Ilaria Mastrorosa, Giulia Matusali, Valentina Mazzotta, Paola Mencarini, Silvia Meschi, Francesco Messina, Sibiana Micarelli, Giulia Mogavero, Marzia Montalbano, Chiara Montaldo, Silvia Mosti, Silvia Murachelli, Maria Musso, Michela Nardi, Assunta Navarra, Martina Nocioni, Pasquale Noto, Roberto Noto, Alessandra Oliva, Ilaria Onnis, Sandrine Ottou, Claudia Palazzolo, Emanuele Pallini, Giulio Palombi, Carlo Pareo, Virgilio Passeri, Federico Pelliccioni, Giovanna Penna, Antonella Petrecchia, Ada Petrone, Elisa Pianura, Carmela Pinnetti, Maria Pisciotta, Pierluca Piselli, Silvia Pittalis, Agostina Pontarelli, Costanza Proietti, Vincenzo Puro, Paolo Migliorisi Ramazzini, Alessia Rianda, Gabriele Rinonapoli, Silvia Rosati, Dorotea Rubino, Martina Rueca, Alberto Ruggeri, Alessandra Sacchi, Alessandro Sampaolesi, Francesco Sanasi, Carmen Santagata, Alessandra Scarabello, Silvana Scarcia, Vincenzo Schininà, Paola Scognamiglio, Laura Scorzolini, Giulia Stazi, Giacomo Strano, Chiara Taibi, Giorgia Taloni, Tetaj Nardi, Roberto Tonnarini, Simone Topino, Martina Tozzi, Francesco Vairo, Alessandra Vergori, Laura Vincenzi, Ubaldo Visco-Comandini, Serena Vita, Pietro Vittozzi, Mauro Zaccarelli, Antonella Zanetti, Mondi, A., Lorenzini, P., Castilletti, C., Gagliardini, R., Lalle, E., Corpolongo, A., Valli, M. B., Taglietti, F., Cicalini, S., Loiacono, L., Di Gennaro, F., D'Offizi, G., Palmieri, F., Nicastri, E., Agrati, C., Petrosillo, N., Ippolito, G., Vaia, F., Girardi, E., Capobianchi, M. R., Antinori, A., Zito, S., Abbonizio, M. A., Abdeddaim, A., Agostini, E., Albarello, F., Amadei, G., Amendola, A., Antonica, M. A., Antonini, M., Bartoli, T. A., Baldini, F., Barbaro, R., Bartolini, B., Bellagamba, R., Benigni, M., Bevilacqua, N., Biava, G., Bibas, M., Bordi, L., Bordoni, V., Boumis, E., Branca, M., Buonomo, R., Busso, D., Camici, M., Campioni, P., Canichella, F., Capone, A., Caporale, C., Caraffa, E., Caravella, I., Carletti, F., Cataldo, A., Cerilli, S., Cerva, C., Chiappini, R., Chinello, P., Cianfarani, M. A., Ciaralli, C., Cimaglia, C., Cinicola, N., Ciotti, V., Colavita, F., Cristofaro, M., Curiale, S., D'Abramo, A., Dantimi, C., De Angelis, A., De Angelis, G., De Palo, M. G., De Zottis, F., Di Bari, V., Di Lorenzo, R., Di Stefano, F., Donno, D., Evangelista, F., Faraglia, F., Farina, A., Ferraro, F., Fiorentini, L., Frustaci, A., Fusetti, M., Galati, V., Galli, P., Garotto, G., Gaviano, I., Tekle, S. G., Giancola, M. L., Giansante, F., Giombini, E., Granata, G., Greci, M. C., Grilli, E., Grisetti, S., Gualano, G., Iacomi, F., Iaconi, M., Iannicelli, G., Inversi, C., Lamanna, M. E., Lanini, S., Lapa, D., Lepore, L., Libertone, R., Lionetti, R., Liuzzi, G., Lucia, A., Lufrani, F., Macchione, M., Maffongelli, G., Marani, A., Marchioni, L., Mariano, A., Marini, M. C., Maritti, M., Mastrobattista, A., Mastrorosa, I., Matusali, G., Mazzotta, V., Mencarini, P., Meschi, S., Messina, F., Micarelli, S., Mogavero, G., Montalbano, M., Montaldo, C., Mosti, S., Murachelli, S., Musso, M., Nardi, M., Navarra, A., Nocioni, M., Noto, P., Noto, R., Oliva, A., Onnis, I., Ottou, S., Palazzolo, C., Pallini, E., Palombi, G., Pareo, C., Passeri, V., Pelliccioni, F., Penna, G., Petrecchia, A., Petrone, A., Pianura, E., Pinnetti, C., Pisciotta, M., Piselli, P., Pittalis, S., Pontarelli, A., Proietti, C., Puro, V., Ramazzini, P. M., Rianda, A., Rinonapoli, G., Rosati, S., Rubino, D., Rueca, M., Ruggeri, A., Sacchi, A., Sampaolesi, A., Sanasi, F., Santagata, C., Scarabello, A., Scarcia, S., Schinina, V., Scognamiglio, P., Scorzolini, L., Stazi, G., Strano, G., Taibi, C., Taloni, G., Nardi, T., Tonnarini, R., Topino, S., Tozzi, M., Vairo, F., Vergori, A., Vincenzi, L., Visco-Comandini, U., Vita, S., Vittozzi, P., Zaccarelli, M., and Zanetti, A.

Subjects
Male, 0301 basic medicine, Time Factors, medicine.medical_treatment, Respiratory System, coronavirus, Infectious and parasitic diseases, RC109-216, Severity of Illness Index, Cohort Studies, 0302 clinical medicine, risk factors, 030212 general & internal medicine, Respiratory disease, General Medicine, Middle Aged, Virus Shedding, Infectious Diseases, symbols, RNA, Viral, Female, Coronavirus, COVID-19, viral clearance, viral shedding, Risk factors, SARS-CoV-2, Cohort study, Adult, Microbiology (medical), medicine.medical_specialty, viral shedding, Coronaviru, 030106 microbiology, Article, NO, 03 medical and health sciences, symbols.namesake, Internal medicine, Severity of illness, medicine, Humans, Poisson regression, Aged, Proportional Hazards Models, Mechanical ventilation, business.industry, Proportional hazards model, COVID-19, Retrospective cohort study, medicine.disease, Respiratory failure, Risk factor, business, viral clearance
Abstract

Background Few data about predictors and outcomes associated with prolonged SARS-CoV-2 RNA shedding (VS) are available. Methods Retrospective study including all patients admitted with COVID-19 in an Italian reference hospital for infectious diseases between March 1 and July 1, 2020. Predictors of viral clearance (VC) and prolonged VS from upper respiratory tract were assessed by Poisson regression and logistic regression analyses. The causal relation between duration of VS and probability of clinical outcomes was evaluated through inverse probability weighted Cox model. Results 536 subjects were included. Median duration of VS from symptoms onset was 18 days (IQR 12-26). The estimated 30-day probability of VC was 70.2% (95%CI:65-75). At multivariable analysis, patients with comorbidities (aIRR = 0.88, p = 0.004), lymphopenia at hospital admission (aIRR = 0.75, p = 0.032) and with moderate/severe respiratory disease (aIRR = 0.42, p 1000 ng/mL at admission (aOR = 1.76, p = 0.035) independently predicted prolonged VS. The achievement of VC doubled the chance of clinical recovery (aHR = 2.17, p

Published
2021

2. Endocarditis and meningitis associated to nape piercing in a young female: a case report

Author

Andrea, Mariano, Raffaella, Pisapia, Amina, Abdeddaim, Chiara, Taibi, Alessia, Rianda, Laura, Vincenzi, and Gianpiero, D'Offizi

Subjects
Adult, Staphylococcus aureus, Time Factors, Bacteremia, Endocarditis, Bacterial, Staphylococcal Infections, Anti-Bacterial Agents, Meningitis, Bacterial, Treatment Outcome, Risk Factors, Humans, Drug Therapy, Combination, Female, Body Piercing, Gentamicins, Oxacillin
Abstract

Body piercing is a social phenomenon on the rise especially among young people. This procedure may be complicated by serious bacterial and viral infections. We report a case of Staphylococcus aureus infective endocarditis and meningitis arising from the site of a nape piercing, after its removal. A 21-year-old Italian female was admitted to hospital with neurological impairment and sepsis. A diagnosis of endocarditis associated with meningitis by S. aureus, complicated by septic emboli in the brain, retina, skin and kidney, was formulated on the basis of modified Duke's criteria. The likely port-of-entry was the site of a nape piercing, removed two months before. In view of the widespread practice of body piercing, provision of correct and timely information concerning the associated serious risks is now imperative. Such information should emphasise the option for antibiotic prophylaxis, and the importance of careful local hygiene, even after piercing removal.

Published
2015

3. Metabolic and Morphologic Disorders in Patients Treated with Highly Active Antiretroviral Therapy since Primary HIV Infection

Author

Vincenzo Galati, Valerio Tozzi, Nicoletta Orchi, Laura Vincenzi, Rita Bellagamba, Vincenzo Puro, Gabriella De Carli, Pasquale Narciso, Chiarina Carvelli, and Gianpiero D'Offizi

Subjects
Adult, Male, medicine.medical_specialty, Time Factors, Adolescent, HIV Infections, Physical examination, General Biochemistry, Genetics and Molecular Biology, Metabolic Diseases, History and Philosophy of Science, Antiretroviral Therapy, Highly Active, Internal medicine, medicine, Humans, In patient, Prospective Studies, medicine.diagnostic_test, business.industry, General Neuroscience, Incidence (epidemiology), Hypertriglyceridemia, Middle Aged, medicine.disease, Regimen, Immunology, Female, Lipodystrophy, business, Body mass index, Dyslipidemia
Abstract

UNLABELLED Our objective was to describe morphologic and metabolic disorders in patients treated with highly active antiretroviral therapy (HAART) since primary HIV infection (PHI). Our method was prospective evaluation of patients with PHI initiating HAART at the time of diagnosis. Outcome measures were: development of hyperglycemia, hypercholesterolemia, hypertriglyceridemia, and of body shape abnormalities indicative of lipodystrophy, assessed through self-reported questionnaires and physical examination. RESULTS From May 1997 to April 2001, 41 patients (35 males) with PHI presented at the National Institute for Infectious Diseases "Lazzaro Spallanzani" in Rome, Italy. A protease inhibitor-including regimen was started in 30 patients, and a nonnucleoside reverse transcriptase-inhibitor in 11. Median interval between enrollment and treatment initiation was 30 days (mean 39, range 10-150). Median HAART duration was 19 months (mean 21.2, range 3-47). Thirty-eight patients had undetectable (less than 80 cp/mL) HIV RNA after a median of 3 months (mean 4.1, range 1-15). Mean CD4 cells count increased from 632/mmc at baseline to 936/mmc at the last follow up. No cases of hyperglycemia (glucose level greater than 110 mg/dL) were observed. After a median of 6 months on HAART, 10 patients developed beyond grade 2 (greater than 240 mg/dL) hypercholesterolemia, 5 developed beyond grade 2 (greater than 400 mg/dL) hypertrygliceridemia, and two developed both. Body mass index did not change significantly. Five patients (12.2%) developed lipodystrophy after a median of 14.5 months (mean 15.3, range 2-30), with an incidence of 7.3 per 100 patient-years. CONCLUSIONS Dyslipidemia and lipodystrophy can occur in patients treated with HAART since PHI. This risk of should be taken into account when considering this early antiretroviral treatment of HIV infection.

Published
2001

4. Molecular characterization of hepatitis A outbreak in the province of Rome, Lazio region, Italy, January-July 2013

Author

Maria R, Capobianchi, Anna Rosa, Garbuglia, Chiara, Agrati, Alessia, Rianda, Pasquale, Noto, Angela, Corpolongo, Maria Adriana, Cataldo, Silvia, Rosati, Paola, Zaccaro, Mariarosaria, Loffredo, Maria Grazia, Pompa, Enrico, Girardi, Paola, Scognamiglio, Giuseppe, Ippolito, and Laura, Vincenzi

Subjects
Adult, Male, Genotype, Immunology, Molecular Sequence Data, Sequence Homology, Biology, Microbiology, Disease Outbreaks, Monophyly, medicine, Cluster Analysis, Humans, Phylogeny, Molecular Epidemiology, Outbreak, Hepatitis A, Sequence Analysis, DNA, medicine.disease, Virology, Hepatitis a virus, Northern italy, Infectious Diseases, Lazio region, Italy, RNA, Viral, Vaccine-preventable diseases, Female, Hepatitis A virus
Abstract

Reduced circulation of hepatitis A virus lead to an increase of susceptible individuals, and outbreaks occurred recently. In Northern Italy an outbreak is ongoing, attributed to a monophyletic genotype IA strain, with mixed frozen berries as probable source. From 01/01/2013 to 07/15/2013, 30 cases were diagnosed at National Institute for Infectious Diseases, Rome, Italy, representing about twice the number of cases in whole 2012. Phylogenetic analysis indicated that most, although not all, infections were attributable to the same monophyletic genotype IA strain identified in the contemporary Northern Italy outbreak. This strain is also very similar to previous isolates from Venezuela.

Published
2013

5. Varicella zoster virus infection presenting as isolated diplopia: a case report

Author

A. Testa, Laura Vincenzi, Raffaella Pisapia, Pasquale Narciso, Alessandra Oliva, Simonetta Galgani, Amina Abdeddaim, Alessia Rianda, and Andrea Mariano

Subjects
Adult, Male, medicine.medical_specialty, Herpesvirus 3, Human, viruses, Anti-Inflammatory Agents, Case Report, Varicella-zoster virus infection, medicine.disease_cause, Antiviral Agents, Herpes Zoster, Diagnosis, Differential, Medical microbiology, Diplopia, Medicine, Humans, integumentary system, business.industry, Trochlear nerve, Varicella zoster virus, virus diseases, Trochlear Nerve Diseases, Dermatology, Rash, Virology, eye diseases, Infectious Diseases, Molecular Diagnostic Techniques, Molecular diagnosis, medicine.symptom, Differential diagnosis, business, Cranial nerve palsy, Clinical awareness
Abstract

Background Involvement of trochlear nerve during Varicella Zoster Virus (VZV) Infection has been rarely described, and always in association with skin rash. Case presentation We describe the case of a patient with VZV infection presenting as isolated diplopia due to fourth cranial nerve palsy. The diagnosis has been obtained through the application of a standardized molecular diagnostic panel, and diplopia resolved after specific antiviral and corticosteroid therapy. Conclusion This case evidences that clinicians should be aware of atypical VZV infection, even in the absence of the typical skin rash.

Published
2012

6. Correlation between enzyme-linked immunosorbent assay and immunofluorescence assay with lytic antigens for detection of antibodies to human herpesvirus 8

Author

Simone Topino, Ivano Mezzaroma, Maria Caterina Sirianni, Laura Vincenzi, Massimo Andreoni, and Emanuele Nicastri

Subjects
Microbiology (medical), Adult, Male, AIDS-Related Opportunistic Infections, Clinical Biochemistry, Immunology, Fluorescent Antibody Technique, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Antibodies, Viral, Antigen, medicine, Tumor Cells, Cultured, Immunology and Allergy, Humans, Antigens, Viral, Sarcoma, Kaposi, Aged, chemistry.chemical_classification, Aged, 80 and over, biology, medicine.diagnostic_test, Antibody titer, Middle Aged, Virology, Molecular biology, Enzyme, Lytic cycle, chemistry, Immunoassay, Herpesvirus 8, Human, biology.protein, Female, Microbial Immunology, Antibody
Abstract

The purpose of this study was to evaluate, in Kaposi's sarcoma patients, the correlation between antibody titers to the lytic antigens of human herpesvirus 8, as assessed by immunofluorescence assay, and values obtained by an enzyme immunoassay. The methods showed a stringent correlation, r = 0.625 ( P < 0.001).

Published
2001

7. Decay of HIV type 1 DNA and development of drug-resistant mutants in patients with primary HIV type 1 infection receiving highly active antiretroviral therapy

Author

Elisabetta Riva, Guido Antonelli, Ferdinando Dianzani, Laura Vincenzi, Vincenzo Galati, Valerio Tozzi, Ombretta Turriziani, Pasquale Narciso, Mauro Pistello, Patrizia Isola, and Gianpiero D'Offizi

Subjects
Adult, Male, HAART, Adolescent, Anti-HIV Agents, Immunology, HIV Infections, Viremia, Human immunodeficiency virus, HIV, Antiviral therapy, Virus, Proviruses, Acquired immunodeficiency syndrome (AIDS), Antiretroviral Therapy, Highly Active, Virology, Drug Resistance, Viral, medicine, Humans, biology, virus diseases, Viral Load, Provirus, medicine.disease, biology.organism_classification, Genes, pol, Infectious Diseases, Viral replication, DNA, Viral, Mutation, Lentivirus, HIV-1, Leukocytes, Mononuclear, RNA, Viral, Female, Viral disease, Viral load
Abstract

The present study was aimed at describing the effect of highly active antiretroviral therapy (HAART) in 10 patients with primary HIV infection (PHI). Clearance rates of HIV RNA and HIV DNA in peripheral blood as well as the preexistence and the emergence of drug-resistant strains of HIV were determined over 52 weeks of treatment. The data indicate that HAART is able to induce a suppression of plasma viral load together with a significant decrease, but not a suppression, of peripheral blood mononuclear cell-associated proviral DNA in PHI subjects. Analysis of drug-resistant strains revealed that three PHI patients, showing a complete virologic response, developed mutations in the pol gene, thus suggesting that a persistent residual virus replication exists despite a sustained suppression of plasma viremia.

Published
2001

8. Kaposi's Sarcoma–Associated Herpesvirus/Human Herpesvirus 8 Is Not Detectable in Peripheral Blood Mononuclear Cells of the Relatives of Sporadic KS Patients

Author

Maria Caterina Sirianni, Francesca Cottoni, Carlo D. Baroni, Alberto Faggioni, Luigi Ruco, Decio Cerimele, Antonella Stoppacciaro, Roberta Santarelli, Stefania Uccini, Antonio Angeloni, Laura Vincenzi, Maria Vittoria Masala, and Emanuela Pilozzi

Subjects
Adult, Aged, 80 and over, Male, business.industry, Cell Biology, Dermatology, Middle Aged, medicine.disease_cause, Biochemistry, Peripheral blood mononuclear cell, Virology, DNA, Viral, Herpesvirus 8, Human, Immunology, Leukocytes, Mononuclear, Humans, Medicine, Female, Kaposi's sarcoma-associated herpesvirus, business, Sarcoma, Kaposi, Molecular Biology, Human herpesvirus, Aged
Published
1997

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