1. Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
- Author
-
Giner Lamia, Joaquín, Vinuesa, Pablo, Betancor, Laura, Silva, Claudia, Bisio, Julieta, Soleto, Lorena, Chabalgoity, José A., Puente, José Luis, Soncini, Fernando Carlos, Garcia Vescovi, Eleonora, García Del Portillo, Francisco, Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo, European Commission, Ministerio de Economía y Competitividad (España), Consejo Nacional de Ciencia y Tecnología (México), Universidad de la República (Uruguay), Agencia Estatal de Investigación (España), Consejo Superior de Investigaciones Científicas (España), García del Portillo, Francisco, and García del Portillo, Francisco [0000-0002-4120-0530]
- Subjects
Adult ,0106 biological sciences ,Salmonella ,Genomic Islands ,lcsh:QH426-470 ,Virulence Factors ,Type-III effectors ,lcsh:Biotechnology ,Virulence ,Biology ,medicine.disease_cause ,01 natural sciences ,Genome ,purl.org/becyt/ford/1 [https] ,Young Adult ,03 medical and health sciences ,Plasmid ,lcsh:TP248.13-248.65 ,Genetics ,medicine ,Humans ,purl.org/becyt/ford/1.6 [https] ,Phylogeny ,030304 developmental biology ,Comparative genomics ,0303 health sciences ,Infant, Newborn ,Correction ,Salmonella enterica ,Subspecies diarizonae ,Genomics ,biology.organism_classification ,Pathogenicity island ,3. Good health ,lcsh:Genetics ,Invasive human infections ,Virulence genes ,Salmonella Infections ,Female ,Genome, Bacterial ,Cation transport ,010606 plant biology & botany ,Biotechnology ,Research Article - Abstract
[Background] Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are currently unknown., [Results] We report here the genome features of two diarizonae strains, SBO13 and SBO27, isolated from endocervical tissue collected post-abortion and from cerebrospinal fluid of a newborn child, respectively, in the city of Santa Cruz, Bolivia. Although isolated six years apart, SBO27 in 2008 and SBO13 in 2014, both strains belong to the same sequence type 1256 (ST1256) and show a high degree of genome conservation sharing more than 99% of their genes, including the conservation of a ~ 10 kb plasmid. A prominent feature of the two genomes is the presence of 24 genomic islands (GIs), in addition to 10 complete Salmonella pathogenicity islands (SPI) and fragments of SPI-7, a pathogenicity island first reported in the human-adapted serovar Typhi. Some of the GIs identified in SBO13 and SBO27 harbor genes putatively encoding auto-transporters involved in adhesion, lipopolysaccharide modifying enzymes, putative toxins, pili-related proteins, efflux pumps, and several putative membrane cation transport related-genes, among others. These two Bolivian isolates also share genes encoding the type-III secretion system effector proteins SseK2, SseK3 and SlrP with other diarizonae sequence types (ST) mainly-associated with infections in humans. The sseK2, sseK3 and slrP genes were either absent or showing frameshift mutations in a significant proportion of genomes from environmental diarizonae isolates., [Conclusions] The comparative genomic study of two diarizonae strains isolated in Bolivia from human patients uncovered the presence of many genes putatively related to virulence. The statistically-significant acquisition of a unique combination of these functions by diarizonae strains isolated from humans may have impacted the ability of these isolates to successfully infect the human host., This work was supported by grants 215RT-0493 from Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo (CYTED), BIO2016–77639-P (AEI/FEDER, UE) and PCIN-2016-082, from Spanish Ministry of Economy and Competitiveness and European Regional Development Funds (FEDER), awarded to FGdP; and, grants DGAPA IN213516, CONACyT 239659 and FC-2015-2/950, awarded to JLP. PV gratefully acknowledges funding grants DGAPA-PAPIIT IN211814 and IN206318 and CONACyT FC-2015-2/879. Other supporting grants were from Comisión Sectorial de Investigación Científica (CSIC), Universidad de la República (Proyecto Grupos I + D 2014), awarded to LB. CS was supported by CONACyT FC-2015-2/879., We acknowledge support of the publication fee by the CSIC Open Access Support Initiative through its Unit of Information Resources for Research (URICI)
- Published
- 2019