Search

• • Slc7a5 knockdown results in 34 differentially regulated genes. • • B1a but not B2 cells are depleted when Slc7a5 is knocked down in adult mice. • • Slc7a5-deficient bone marrow B1a cells fail to develop due to dysregulation of the mTOR signaling pathway. Slc7a5 is an important amino acid transporter that is highly expressed in metabolically active and rapidly proliferating cells. To explore the effect of Slc7a5 on adult B cell development, we conditionally deleted Slc7a5 in murine B cells and observed a significant reduction of B1a cells. In contrast to PI3K-Akt pathway activation, activity of the mTOR pathway was decreased. This may result from intracellular amino acid starvation in Slc7a5 knockdown (Slc7a5 KD) bone marrow B cells, thereby dampening B1a development. RNA-seq analysis demonstrated increased translation and reduced proliferation in Slc7a5 KD bone marrow B cells. Overall, the results of our study highlight the importance of Slc7a5 in peritoneal B1a cell development. [ABSTRACT FROM AUTHOR]