1. The Apyrase gene of the vector mosquito, Aedes aegypti, is expressed specifically in the adult female salivary glands.
- Author
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Smartt CT, Kim AP, Grossman GL, and James AA
- Subjects
- Aedes enzymology, Amino Acid Sequence, Animals, Apyrase biosynthesis, Apyrase chemistry, Base Sequence, Blotting, Northern, Cloning, Molecular, DNA Primers chemistry, DNA, Complementary chemistry, Female, Gene Expression Regulation, Enzymologic, Immunoblotting, Insect Vectors enzymology, Male, Molecular Sequence Data, RNA analysis, RNA Probes, Salivary Glands enzymology, Sex Characteristics, Aedes genetics, Apyrase genetics, Genes, Insect, Insect Vectors genetics
- Abstract
The yellow fever mosquito, Aedes aegypti, expresses a gene, Apyrase (Apy), that encodes an ATP-diphosphohydrolase. The product of this gene is a secreted enzyme that facilitates hematophagy by preventing platelet aggregation in the host. Apy gene expression is limited to the cells of the distal-lateral and medial lobes of the adult female salivary glands. Apyrase protein levels, detectable by antibodies, peak in the salivary glands about 4 days after adult emergence and remain high after a blood meal. Primary sequence analysis of a genomic clone encoding apyrase reveals a unique TAAATA sequence and seven introns, as well as other conserved features of eukaryotic genes. The temporal, sex- and tissue-specific expression of the Apy gene is consistent with its role as encoding a platelet anti-aggregation factor that functions to facilitate hematophagy and decrease probing time.
- Published
- 1995
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