1. Optimization of Nannochloropsis oculata growth using the response surface method
- Author
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Pauline Spolaore, Arsène Isambert, Claire Joannis-Cassan, Elie Duran, Laboratoire de Génie des Procédés et Matériaux - EA 4038 (LGPM), CentraleSupélec, CIERM, Université Paris-Sud - Paris 11 (UP11), EVAFLOR (FRANCE), and Ecole Centrale Paris (FRANCE)
- Subjects
Optimization ,0106 biological sciences ,Central composite design ,General Chemical Engineering ,Analytical chemistry ,01 natural sciences ,Inorganic Chemistry ,03 medical and health sciences ,010608 biotechnology ,Optimum growth ,Génie chimique ,Statistical analysis ,Nannochloropsis oculata ,Growth rate ,Nannochloropsis ,Génie des procédés ,central composite design ,Waste Management and Disposal ,030304 developmental biology ,0303 health sciences ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Organic Chemistry ,Environmental engineering ,Pollution ,microalga ,Fuel Technology ,Aeration rate ,[CHIM.OTHE]Chemical Sciences/Other ,optimization ,Intensity (heat transfer) ,Microalga ,Nannochlorospis ,Biotechnology - Abstract
International audience; Optimization of Nannochloropsis oculata growth was undertaken using the response surface method. A central composite design was defined to study the effects of temperature, pH, incident light intensity and aeration rate on the maximum growth rate of the microalga. Using statistical analysis, the first model calculated to fit results was twice improved. The final model obtained was used to clarify the effects of each factor and their interactions on the growth of Nannochloropsis oculata. The optimum growth conditions of this microorganism were also estimated as 21 degrees C, 52 mu mol photons m(-2) s(-1), pH 8.4 and 14.7 VVH of aeration rate. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, 0.0359 h(-1), is the best reported in this study.
- Published
- 2006
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