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The use of heart rate (HR) monitors for horse training assessment under field conditions has been expanded because its use facilitates the understanding of horse fitness levels. Work has been carried out to determine the HR, velocity (V) and exercise time for pull and helper horses submitted to the Vaquejada simulation test (VST). The test was used on 70 Quarter Horses that had trained and competed for more than 6 months. The VST consists of two horses, a puller and a helper, and a bull, all running on a soft sand track. A race cycle consists of three runs with the bull. Pull horses run one cycle and helper horses run two cycles. A Polar V800-GPS HR monitor for equines was used to measure several parameters: time, V and HR. The results were analysed by ANOVA and a Tukey test with P set at 5%. It was shown that pull horses developed an HRmax of ∼200 bpm and an HRmin ∼110 bpm, both higher than the helper horses (P<0.05). However, the HRmed of pull horses (∼150 bpm) and helper horses (∼139 bpm) in C-2 trials were not substantially different from one another (P>0.05). Additionally, both groups of horses had similar maximum (∼8.90 m/s) and average (∼1.87 m/s) velocities (P>0.05). In analysing the distribution of HR among five rate categories, we determined that pull horses presented a higher percentage of their HR above 170 bpm in comparison to helper horses (P<0.05). It was concluded that, based on VST results, pull and helper horses presented different HRmax, HRmed and HRmin values, even when V and duration were similar, and that pull horses perform more intensive work than helper horses. [ABSTRACT FROM AUTHOR]