1. [Adsorptive immobilization of rhodococcal cells in hydrophobized derivatives of wide-pore polyacrylamide cryogel].
- Author
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Kuyukina MS, Ivshina IB, Rubtsova EV, Ivanov RV, and Lozinskiĭ VI
- Subjects
- Alkanes chemistry, Bacterial Adhesion, Biocatalysis, Cells, Immobilized, Hydrophobic and Hydrophilic Interactions, Industrial Oils, Microbial Viability, Porosity, Rhodococcus chemistry, Acrylic Resins chemistry, Aerobiosis physiology, Cryogels chemistry, Oils metabolism, Oxygen metabolism, Rhodococcus physiology
- Abstract
Adsorption of Rhodococcus ruber cells on columns with polyacrylamide cryogel (CryoPAAG) partially hydrophobized by different quantities (0.2, 1, and 5 mol %) of chemically grafted n-dodecane residues has been studied. The adsorption capacity (1.1 x 10(9) cells/g) of gel carrier for rhodococcal cells and the optimal content (1 mol %) of hydrophobizing groups were determined. The respirometric method showed the high catalytic activity and functional stability of immobilized bacterial cells. Respiratory activity of immobilized rhodococci in the presence of a model mixture of oil hydrocarbons exceeded the respective parameter for free cells by 12-17%. Viability of rhodococcal cells adsorptionally fixed in hydrophobized cryoPAAG was maintained at a level of 93-95% after a half-year period of storage. The results may be used for development of immobilized biocatalyst for directed transformation of hydrocarbon compounds and biological purification of oil-polluted water.
- Published
- 2011