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Ecological stoichiometry (C:N:P ratios) in soil plays an important role in ecosystem dynamics and functioning; however, its relationship with below-ground microbial diversity following afforestation remains poorly understood. To illustrate the linkage of C, N, and P in soil and microbial biomass, and the effect on the soil bacterial community, soil samples were collected from farmland and from three afforested land, namely Robinia pseudoacacia L., Caragana korshinskii Kom, and abandoned land, which have been arable for the past 40 years. Quantitative PCR and Illumina sequencing of the 16S rRNA genes were used to analyze soil bacterial abundance, diversity, and composition. Additionally, soil properties and C, N, and P levels in soil and microbial biomass were estimated. The results revealed that C, N, and P levels in soil and microbial biomass increased following afforestation, with a significant correlation observed, especially for the N:P ratio. Additionally, a rise in 〈alpha〉- and 〈beta〉-diversity of soil bacteria was observed in response to afforestation, and was linked to soil C:P and N:P ratios. Soil bacterial phyla with high relative abundance (relative abundance > 1%) across all samples showed inconsistent directional trends in the composition response after afforestation. An increased abundance of Proteobacteria, Acidobacteria, and Nitrospirae were observed, while the abundance of Chloroflexi was opposite (P < 0.05) following afforestation. Those were largely influenced by changes in the N:P ratio in soil and microbial biomass. Taken together, significantly increased N:P ratio in soil reflected the deficient P that would be limited for increased microbial biomass and diversity. Thus, P status should be an important factor for sustainable restoration of the forest during the process of afforestation. [ABSTRACT FROM AUTHOR]

Abstract C:N:P stoichiometry in the plants and soils is an important indicator of biogeochemical cycles and functioning in ecosystems; however, the response of plant growth to ecological stoichiometry following afforestation remains unclear. To illustrate the C:N:P stoichiometry connections between the plants and soils and their effects on the growth of plants, soil and plant samples were collected from one farmland site and four Robinia pseudoacacia (RP) forests that were planted for 10, 15, 25, and 35 years. Plant community characteristics, growth of overstory trees and understory plants, physical and chemical properties of the soil, and C:N:P stoichiometry in plants and soil were measured. The results revealed that soil organic C, total N, total P, dissolved organic N, C:P and N:P ratios, and water content significantly increased, whereas soil bulk density, available P, and pH decreased with afforestation age. Leaf C:P and N:P ratios in RP were significantly higher than that in understory biomass because P concentrations gradually decreased in RP leaves and increased in understory biomass. The C, N, and P contents and stoichiometry in the soils and plants were significantly correlated, particularly for N:P ratio that can be used to reveal close coupling between plant and soil nutrients. The N:P ratio in RP leaf and understory biomass increased with afforestation age and varied from 14.44 to 20.81 and 7.59 to 10.96, respectively, suggesting that P limitation gradually increased in RP, and N limitation gradually declined in understory biomass. Furthermore, the responses of overstory trees and understory plant growth to N:P ratios were contradictory, because of their different nutrient acquisition patterns and nutrient-use efficiencies. Therefore, this finding provides evidence that the strong correlation between the plants and soils were tightly coupled to N and P concentrations and N:P ratios, and therefore, have the potential to influence the growth of forests on the Loess Plateau. Graphical abstract Unlabelled Image Highlights • Robinia pseudoacacia afforestation improved soil and plant community characteristics. • Leaf C:P and N:P ratios were higher for Robinia pseudoacacia than for understory plants. • N:P ratios indicate a tight coupling between soil and plant. • P limitation increased in Robinia pseudoacacia and N limitation declined in understory biomass. • N:P ratios negatively correlated with Robinia pseudoacacia growth, but positively correlated with understory biomass. [ABSTRACT FROM AUTHOR]

The goal of this study was to determine the dynamics of physicochemical properties and biological activities of soil, and their relationship following afforestation. Soil samples were collected at 0–10 cm from five Pinus tabulaeformis plantation forests restored for 15, 25, 30, 45, and 70 years, as well as a millet ( Setaria italica ) farmland in the Damaiji catchment area. These five afforested lands were converted from similar farmlands. The activities of catalase (CAT), saccharase (SAC), urease (URE) and alkaline phosphatase (ALP), microbial biomass, soil water content, pH, soil bulk density, soil organic carbon (SOC), total nitrogen (TN), ammonium nitrogen, nitrate nitrogen, total phosphorus (TP), and available phosphorus (AP) were measured. The results revealed that the contents of SOC, TN, AP, microbial biomass C, N, and P, CAT, SAC, URE, and ALP in the P . tabulaeformis forest soil were significantly higher than those in the farmland by 64.97%–262.02%, 75.44%–254.28%, 46.63%–114.21%, 125.95%–554.83%, 101.35%–464.21%, 15.80%–167.35%, 22.57%–49.95%, 96.78%–145.73%, 6.98%–56.08% and 89.15%–177.89%, respectively. The soil properties, microbial biomass, enzymatic activities, and C:P and N:P ratios in soil and microbial biomass improved with increasing plantation chronosequence. Variations in C:N:P stoichiometry and higher C:P and N:P ratios in the soil and microbial biomass revealed the P limitation. Simultaneously, N:P ratios included more serviceable information that reflected the relationship between soil and microbes. Soil enzymatic activities had a high correlation with soil nutrient cycling and could be indicators of soil fertility status, particularly for ALP. The significant correlation between SOC, TN, enzymatic activities, and microbial biomass revealed that the substrate availability of carbon and nitrogen could influence the activity of soil enzymes and microorganisms. This study demonstrated that soil enzymatic activities and microorganisms respond to the process of afforestation and hence have the potential to affect nutrient balance and quality of soil. [ABSTRACT FROM AUTHOR]

Temporal variation in soil enzyme activities has important significance for soil quality after afforestation. This study investigated the temporal variation in soil enzyme activities and their response to changes in soil properties following afforestation. Soil samples were collected during different periods of vegetation growth at sites in the Loess Plateau with different land use types: 40 year-old Robinia pseudoacacia L. (RP40a), Caragana Korshinskii Kom (CK40a), and abandoned land (AL40a), as well as millet ( Setaria italica ) farmland (FL). Activities of four enzymes (catalase, saccharase, urease and alkaline phosphatase) that are involved in C, N, P cycling and soil water content (SWC), dissolved organic carbon (DOC), dissolved organic nitrogen (DON), and available phosphorus (AP) were measured. The results revealed that temporal variation and land-use have significant effects on the patterns of SWC, DOC, and DON, although not on that of AP. In addition, the activities of soil enzymes were highest in June, indicating that there are differences in the temporal variation of soil enzyme activities within a given land-use type, especially for alkaline phosphatase. The consistent ranking of soil enzyme activities was also significantly increased by afforestation. Furthermore, nonmetric multidimensional scaling analysis showed that the influence degree of afforestation on activities of the four enzymes was higher than that of temporal variation. Significant correlations between soil enzyme activities and soil properties indicated that soil enzyme activities are closely related to soil nutrients dynamics, particularly with respect to DON. The present study accordingly demonstrates that soil enzyme activities respond to farmland-to-forest conversion and hence have the potential to affect soil qualities in the Loess Plateau. [ABSTRACT FROM AUTHOR]

The afforestation of abandoned land could offer opportunities to sequester soil organic carbon (SOC), promote nutriment elements cycling, improve plant diversity in the plantation understory and provide ecosystem services. The objectives of this study were to identify plant diversity in the plantation understory, quantify the changes in SOC and total nitrogen (TN) storage in deep soil, assess the SOC, TN, and total phosphorus (TP) stoichiometries, and investigate their relationships in the Loess Plateau Region (LPR) undergoing long-term afforestation. Soil samples were collected at a soil depth of 0–200 cm under 30-yr old Robinia pseudoacacia L. and adjacent abandoned sites, and SOC, TN and TP were determined in different soil depth. Additionally, plant composition and diversity in the plantation understory were evaluated. The results showed that land subjected to long-term afforestation had greater plant coverage, plant density, richness index ( R ) and Shannon–Wiener diversity ( H ) compared to abandoned land communities ( P < 0.05). SOC, TN and TP contents in afforested sites were significantly increased in surface soil (0–30 cm) as well as in the underlying soil (100 cm) compared to the corresponding abandoned land sites ( P < 0.05) in most cases. Meanwhile, SOC, TN, and TP stoichiometry in afforested areas were higher than those of abandoned lands and significantly related to understory vegetation diversity ( P < 0.05). In addition, lands subjected to long-term afforestation effectively increased SOC and TN storages compared to abandoned land at soil depths of 0–30 cm and 100–200 cm and were also significantly related to understory vegetation diversity ( P < 0.05). These findings demonstrating that afforestation not only affects SOC and TN stocks in surface soil, but also strongly influences that in deep soil. And it is also indicating that long-term afforestation could greatly affect soil R CN , R CP , and R NP ratios. [ABSTRACT FROM AUTHOR]