6 results on '"Upadhyay, Neha"'
Search Results
2. Fabrication, characterization and practical efficacy of Myristica fragrans essential oil nanoemulsion delivery system against postharvest biodeterioration.
- Author
-
Das S, Singh VK, Dwivedy AK, Chaudhari AK, Upadhyay N, Singh A, Deepika, and Dubey NK
- Subjects
- Animals, Aspergillus flavus drug effects, Germination drug effects, Lethal Dose 50, Lipid Peroxidation drug effects, Male, Mice, Oils, Volatile chemistry, Oils, Volatile isolation & purification, Oryza drug effects, Oryza microbiology, Plant Extracts chemistry, Plant Extracts isolation & purification, Seeds drug effects, Seeds microbiology, Aflatoxins antagonists & inhibitors, Antifungal Agents pharmacology, Myristica chemistry, Oils, Volatile pharmacology, Plant Extracts pharmacology
- Abstract
The present study deals with encapsulation of Myristica fragrans essential oil (MFEO) into chitosan nano-matrix, their characterization and assessment of antimicrobial activity, aflatoxin inhibitory potential, safety profiling and in situ efficacy in stored rice as environment friendly effective preservative to control the postharvest losses of food commodities under storage. Surface morphology of MFEO-chitosan nanoemulsion as well as encapsulation of MFEO was confirmed through SEM, FTIR and XRD analysis. In vitro release characteristics with biphasic burst explained controlled volatilization from nanoencapsulated MFEO. Unencapsulated MFEO exhibited fungitoxicity against 15 food borne molds and inhibited aflatoxin B
1 secretion by toxigenic Aspergillus flavus LHP R14 strain. In contrast, nanoencapsulated MFEO showed better fungitoxicity and inhibitory effect on aflatoxin biosynthesis at lower doses. In situ efficacy of unencapsulated and nanoencapsulated MFEO on stored rice seeds exhibited effective protection against fungal infestation, aflatoxin B1 contamination, and lipid peroxidation. Both the unencapsulated and nanoencapsulated MFEO did not affect the germination of stored rice seeds confirming non-phytotoxic nature. In addition, negligible mammalian toxicity of unencapsulated MFEO (LD50 = 14,289.32 μL/kg body weight) and MFEO loaded chitosan nanoemulsion (LD50 = 9231.89 μL/kg body weight) as revealed through favorable safety profile recommend the industrial significance of nanoencapsulated MFEO as an effective green alternative to environmentally hazardous synthetic pesticides for protection of food commodities during storage., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
3. Antimicrobial activity, antiaflatoxigenic potential and in situ efficacy of novel formulation comprising of Apium graveolens essential oil and its major component.
- Author
-
Das S, Singh VK, Dwivedy AK, Chaudhari AK, Upadhyay N, Singh A, Deepika, and Dubey NK
- Subjects
- Animals, Anti-Infective Agents toxicity, Chromatography, High Pressure Liquid, Male, Mice, Oils, Volatile toxicity, Aflatoxins antagonists & inhibitors, Anti-Infective Agents pharmacology, Apium chemistry, Oils, Volatile pharmacology
- Abstract
The present study reports the formulation of Apium graveolens essential oil (AGEO) with its major components linalyl acetate (LA) and geranyl acetate (GA) (1:1:1) as a novel green preservative for protection of postharvest food commodities from fungal infestations, aflatoxin B
1 (AFB1 ) secretion, free radical generation and lipid peroxidation. The essential oil based novel formulation displayed considerable inhibitory action against fourteen food borne molds responsible for deterioration of stored food commodities, in addition to the most toxigenic strain of Aspergillus flavus (AFLHPR14) isolated from fungal and aflatoxin contaminated rice seeds. The observed higher efficacy of designed formulation was due to the synergistic action of essential oil and its major components. Fungal plasma membrane was recorded as the possible target site of antifungal action of the formulation as revealed through reduction in membrane ergosterol content, increased intracellular propidium iodide (PI) fluorescence and enhanced leakage of cellular ions (sodium, potassium, calcium) and 260, 280 nm absorbing materials. Further, inhibition of methylglyoxal (an aflatoxin inducer) confirmed the aflatoxin inhibitory potential of novel formulation based on essential oil and its major components. High antioxidant potential as observed through DPPH and ABTS·+ radical scavenging assay, improved phenolic content, considerable inhibition of lipid peroxidation in stored rice seeds, in situ efficacy on AFB1 inhibition in food system under storage container system, acceptable sensorial characteristics and favorable safety profile during animal trials suggest the recommendation of the designed formulation for large scale application as green preservative by food and agriculture based industries against fungal and aflatoxin contamination of stored commodities., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
4. Facile Fabrication of Nanoformulated Cinnamomum glaucescens Essential Oil as a Novel Green Strategy to Boost Potency Against Food Borne Fungi, Aflatoxin Synthesis, and Lipid Oxidation.
- Author
-
Tiwari, Shikha, Upadhyay, Neha, Singh, Bijendra Kumar, Singh, Vipin Kumar, and Dubey, Nawal Kishore
- Subjects
- *
CINNAMOMUM , *LIPIDS , *BLACK cumin , *AFLATOXINS , *ESSENTIAL oils , *BIOPOLYMERS , *FOOD preservatives - Abstract
The study presents chitosan nanoemulsion encapsulating Cinnamomum glaucescens essential oil (CGEO) as a novel green preservative against food born fungi and aflatoxin B1 production in food system. GC–MS analysis suggested 1,8-cineole (46.56%), safrole (17.64%) as major and terpineol (5.88%), 4-terpineol (4.68%), β-pinene (2.08%), β-thujene (2.03%), and linalool (2.02%) as minor components of CGEO. SEM, FTIR, and XRD analysis confirmed the successful encapsulation of CGEO in chitosan biopolymer (CGEO-Nm). Thermogravimetric analysis (TGA) of CGEO-Nm showed improved thermal stability of encapsulated CGEO in comparison to unencapsulated CGEO. Encapsulation remarkably improved antifungal and antiaflatoxigenic efficacy of CGEO-Nm (minimum inhibitory concentration (MIC) = 0.9 µL/mL and MAIC = 0.8 µL/mL) in comparison to unencapsulated CGEO (MIC = 1.50 µL/mL and MAIC = 1.25 µL/mL). Impairment in ergosterol biosynthesis, leakage of vital cellular ions, as well as 260 nm (DNA and RNA) and 280 nm (protein) absorbing materials confirmed cell membrane as antifungal target site for CGEO-Nm. Reduction in methylglyoxal level suggested novel antiaflatoxigenic mechanism of action. The investigation showed enhanced antioxidant potency of CGEO after encapsulation (IC50 value 5.15 µL/mL and 10.45 µL/mL, respectively, for ABTS and DPPH assays). Furthermore, CGEO-Nm was also found to have remarkable in vivo efficacy as it inhibited lipid peroxidation and mineral loss in black cumin seeds without altering its sensorial attributes. CGEO-Nm also revealed mammalian safety (LD50 = 9132.642 mg/kg) as determined through acute oral toxicity assay. Thus, the findings recommend the applicability of CGEO-Nm as novel green preservative to enhance the shelf life of food commodities along with the maintenance of nutritional characteristics. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
5. Chemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based on in vitro and in situ efficacy and favorable safety profile on mice
- Author
-
Tiwari, Shikha, Upadhyay, Neha, Singh, Bijendra Kumar, Singh, Vipin Kumar, and Dubey, Nawal Kishore
- Subjects
ESSENTIAL oils ,AFLATOXINS ,SPICES ,ASPERGILLUS flavus ,MICE ,PYRUVALDEHYDE - Abstract
Present study deals with the efficacy of nanoencapsulated Homalomena aromatica essential oil (HAEO) as a potent green preservative against toxigenic Aspergillus flavus strain (AF-LHP-NS 7), storage fungi, AFB
1 , and free radical-mediated deterioration of stored spices. GC–MS analysis revealed linalool (68.51%) as the major component of HAEO. HAEO was encapsulated into chitosan nanomatrix (CS-HAEO-Ne) and characterized through SEM, FTIR, and XRD. CS-HAEO-Ne completely inhibited A. flavus growth and AFB1 biosynthesis at 1.25 μL/mL and 1.0 μL/mL, respectively in comparison to unencapsulated HAEO (1.75 μL/mL and 1.25 μL/mL, respectively). CS-HAEO-Ne caused significant reduction in ergosterol content in treated A. flavus and provoked leakage of cellular ions (Ca+2 , Mg+2 , and K+ ) as well as 260 nm and 280 nm absorbing materials. Depletion of methylglyoxal level in treated A. flavus cells illustrated the novel antiaflatoxigenic efficacy of CS-HAEO-Ne. CS-HAEO-Ne exhibited superior antioxidant efficacy (IC50 (DPPH) = 4.5 μL/mL) over unencapsulated HAEO (IC50 (DPPH) = 15.9 μL/mL) and phenolic content. CS-HAEO-Ne depicted excellent in situ efficacy by inhibiting fungal infestation, AFB1 contamination, lipid peroxidation, and mineral loss with acceptable sensorial profile. Moreover, broad safety paradigm (LD50 value = 7150.11 mg/kg) of CS-HAEO-Ne also suggests its application as novel green preservative to enhance shelf life of stored spices. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
6. Assessment of nanoencapsulated Cananga odorata essential oil in chitosan nanopolymer as a green approach to boost the antifungal, antioxidant and in situ efficacy.
- Author
-
Upadhyay, Neha, Singh, Vipin Kumar, Dwivedy, Abhishek Kumar, Chaudhari, Anand Kumar, and Dubey, Nawal Kishore
- Subjects
- *
AFLATOXINS , *ANTIFUNGAL agents , *ESSENTIAL oils , *CHITOSAN , *FOURIER transform infrared spectroscopy , *PEANUTS , *FOOD storage - Abstract
In this study, a comparative efficacy of Cananga odorata EO (CoEO) and its nanoencapsulated formulation into chitosan nanoemulsion (CoEO-CsNe) against a toxigenic strain of Aspergillus flavus (AF-M-K5) were investigated for the first time in order to determine its efficacy in preservation of stored food from fungal, aflatoxin B 1 (AFB 1) contamination and lipid peroxidation. GC and GC–MS analysis of CoEO revealed the presence of linalool (24.56%) and benzyl acetate (22.43%) as the major components. CoEO was encapsulated into chitosan nanoemulsion (CsNe) through ionic-gelation technique and characterized by High Resolution-Scanning Electron Microscopy (HR-SEM), Fourier Transform Infrared spectroscopy (FTIR), and X-Ray Diffraction (XRD) analysis. The CoEO-CsNe during in vitro investigation against A. flavus completely inhibited the growth and AFB 1 production at 1.0 μL/mL and 0.75 μL/mL, respectively. Additionally, CoEO-CsNe showed improved antioxidant activity against DPPH• and ABTS•+ with IC 50 value 0.93 and 0.72 μL/mL, respectively. Further, CoEO-CsNe suppressed fungal growth, AFB 1 secretion and lipid peroxidation in Arachis hypogea L. during in situ investigation without causing any adverse effect on seed germination. Overall results demonstrated that the CoEO-CsNe has potential of being utilized as a suitable plant based antifungal agent to improve the shelf-life of stored food against AFB 1 and lipid peroxidation mediated biodeterioration. • CoEO was nanoencapsulated into chitosan nanoemulsion (CsNe) through ionic gelation. • CoEO-CsNe was characterized through HR-SEM, FTIR, and XRD analysis. • CoEO-CsNe exhibited enhanced efficacy during in vitro and in situ condition. • CoEO-CsNe may be recommended as green preservative for food application. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.