1. Ratiometric fluorescence and absorbance dual-model immunoassay based on 2,3-diaminophenazine and carbon dots for detecting Aflatoxin B1.
- Author
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Xu, Die, Zhang, Junxiang, Luo, Zhenzhen, Zhao, Yanan, Zhu, Yuanhua, Yang, Hualin, and Zhou, Yu
- Subjects
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FLUORESCENCE , *IMMUNOASSAY , *AFLATOXINS , *LIGHT absorbance , *HORSERADISH peroxidase , *CHEMILUMINESCENCE immunoassay - Abstract
• The fluorescence model has a ratiometric fluorescence signal to improve accuracy. • The product DAP has a yellow color can be directly observed by the naked eye. • This dual-model immunoassay shows high sensitivity and selectivity for AFB1 detection. In this work, a dual-model immunoassay for detecting Aflatoxin B1 (AFB1) was developed based on 2,3-diaminophenazine (DAP) and carbon dots (CDs). Under the catalysis of horseradish peroxidase (HRP), the o -phthalylenediamine (OPD) was oxidized to DAP which had a yellow color and intense fluorescence. The color changes form colorless to yellow was used to design absorbance model immunoassay. Meanwhile, the absorption spectrum of DAP overlapped with the emission spectrum of CDs which caused the fluorescence of CDs to be quenched. The fluorescence changes of DAP and CDs were used to develop ratiometric fluorescence immunoassay. The dual-model immunoassay showed excellent sensitivity with the limits of detection (LODs) of 0.013 ng/mL for fluorescence mode and 0.062 ng/mL for absorbance mode. Meanwhile, both models exhibited great selectivity for AFB1. Additionally, the recovery rates suggested the proposed dual-model immunoassay had great potential in actual samples detection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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