1. Characterization of Adaptive-like γδ T Cells in Ugandan Infants during Primary Cytomegalovirus Infection
- Author
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Cetare Mohsenzadeh-Green, Bing Cai, David Vermijlen, Hugo Soudeyns, Laura Cook, Maria Papadopoulou, Soren Gantt, Jessica Tuengel, Sara Mostafavi, Sibyl Drissler, Gurpreet Aulakh, Peter van den Elzen, Alexandra Maslova, and Sanya Ranchal
- Subjects
Male ,Vγ9negVδ2 ,Cytomegalovirus ,Lymphocyte Activation ,NKG2C ,Cohort Studies ,0302 clinical medicine ,HLA-E ,T-Lymphocyte Subsets ,Uganda ,Prospective Studies ,Gamma delta T cell ,Pathologie maladies infectieuses ,Child ,Intraepithelial Lymphocytes ,0303 health sciences ,education.field_of_study ,medicine.diagnostic_test ,Age Factors ,CMV ,virus diseases ,Receptors, Antigen, T-Cell, gamma-delta ,Flow Cytometry ,QR1-502 ,gammadelta ,3. Good health ,Infectious Diseases ,medicine.anatomical_structure ,Child, Preschool ,Cytomegalovirus Infections ,Vγ8 ,Female ,Gammadelta ,Adult ,gamma delta T cell ,T cell ,Population ,Congenital cytomegalovirus infection ,Receptors, Antigen, T-Cell ,Vδ3 ,Biology ,Microbiology ,Article ,Flow cytometry ,Vδ1 ,03 medical and health sciences ,Virology ,medicine ,Humans ,education ,030304 developmental biology ,Fetus ,CCMV ,immune ontogeny ,T-cell receptor ,Infant, Newborn ,Infant ,medicine.disease ,Immune ontogeny ,cCMV ,Immunology ,Sciences pharmaceutiques ,030215 immunology - Abstract
Gamma-delta (γδ) T cells are unconventional T cells that help control cytomegalovirus (CMV) infection in adults. γδ T cells develop early in gestation, and a fetal public γδ T cell receptor (TCR) clonotype is detected in congenital CMV infections. However, age-dependent γδ T cell responses to primary CMV infection are not well-understood. Flow cytometry and TCR sequencing was used to comprehensively characterize γδ T cell responses to CMV infection in a cohort of 32 infants followed prospectively from birth. Peripheral blood γδ T cell frequencies increased during infancy, and were higher among CMV-infected infants relative to uninfected. Clustering analyses revealed associations between CMV infection and activation marker expression on adaptive-like Vδ1 and Vδ3, but not innate-like Vγ9Vδ2 γδ T cell subsets. Frequencies of NKG2C+CD57+ γδ T cells were temporally associated with the quantity of CMV shed in saliva by infants with primary infection. The public γδ TCR clonotype was only detected in CMV-infected infants, SCOPUS: ar.j, info:eu-repo/semantics/published
- Published
- 2021