1. Induction of cross-reactive antibodies against mimotopes of H5N1 hemagglutinin
- Author
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Song, Huijuan, Luo, Wenxin, Chen, Yingwei, Du, Hailian, Tang, Jixian, Yin, Boyuan, Chen, Yixin, Shih, James Wai-Kuo, and Zhang, Jun
- Subjects
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CROSS reactions (Immunology) , *IMMUNOGLOBULINS , *HEMAGGLUTININ , *PEPTIDES , *EPITOPES , *IMMUNE serums , *AGGLUTINATION , *IMMUNITY , *INFLUENZA A virus, H5N1 subtype - Abstract
Abstract: A sub-library based on peptide mimic 125 was designed and constructed, and 18 phagotopes specifically binding 8H5mAb were isolated. Antisera against three phagotopes, containing peptide 12MH-1, 12MH-5 and 12MH-8 reacted with 3 different H5N1 virus strains, but not with 2 H1N1 and 2 H3N2 viruses by Dot blots. The affinity of 12MH-8 was approximately eight times more than 12MH-1 or 12MH-5 or parent peptide 125. Furthermore, synthesized 12MH-1 and 12MH-8 could block the 8H5mAb binding with 4 H5N1 virus strains via hemagglutinin inhibition. These results suggest that these 3 mimotopes closely mimics the native 8H5 epitopes. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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