1. Assessment of the bioactive phenolic composition of Acer truncatum seed coat as a byproduct of seed oil
- Author
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Jinchi Zhou, Ke Li, Lingguang Yang, Xiang Li, Yujun Liu, Peipei Yin, Qiang Xue, Hang Fan, and Liwei Sun
- Subjects
ABTS ,biology ,DPPH ,010401 analytical chemistry ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Proanthocyanidin ,chemistry ,Acer truncatum ,Polyphenol ,Composition (visual arts) ,Food science ,Phenols ,Gentisic acid ,Agronomy and Crop Science - Abstract
Acer truncatum seed coat is generally abandoned as waste during the process of manufacturing seed oil due to a lack of knowledge of the phytochemical composition of this byproduct. It is well known that brown-colored seed coat is rich in polyphenols; therefore, we investigated the bioactive phenolic composition of A. truncatum seed coat in this study. To obtain an extract containing most bioactive phenolic compounds, extractions in six solutions (water, 40% ethanol, 70% ethanol, 100% ethanol, 70% acetone, and 100% acetone) were compared for their yields, phenolic contents, and antioxidant activities. The 70% ethanol extract, which had the highest yield at 76.38 g extract/100 g dry matter, showed the highest levels of total phenols, total flavonoids, total tannins, and condensed tannins, as well as the highest DPPH and ABTS+ scavenging activities. UPLC-QTOF-MS analyses resulted in identification of 13 phenolic compounds in the 70% ethanol extract, among which five compounds, gentisic acid, naringenin, epicatechin, procyanidin tetramer, and procyanidin pentamer, were identified for the first time in A. truncatum. Five flavonoids and four procyanidin oligomers were the dominant phenolic components, comprising 63.27% and 31.02% of the 13 phenolic compounds, respectively. Correlation analyses revealed that flavonoids and tannins were the major factors responsible for the high antioxidant activities, suggesting that these two components of A. truncatum seed coat could be developed as alternative antioxidants. This is the first known report on the phenolic composition of seed coats in the genus Acer.
- Published
- 2018
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