1. High frequency of transversion mutations in the rice (Oryza sativa L.) mutant population produced by diepoxybutane mutagenesis
- Author
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Tomoki Hoshino, Masanori Watanabe, Tamae Kawakami, Hajime Goto, Masaru Chuba, and Yohei Abe
- Subjects
0106 biological sciences ,0301 basic medicine ,TILLING ,Ethyl methanesulfonate ,Mutant ,Population ,Mutagenesis (molecular biology technique) ,Mutagen ,Plant Science ,Biology ,medicine.disease_cause ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Genetics ,medicine ,education ,Transversion ,Ecology, Evolution, Behavior and Systematics ,Mutation ,education.field_of_study ,030104 developmental biology ,chemistry ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Discovering new genetic mutations is vital for expanding genetic resources for exploring the functions and breeding applications of genes. In this study, we created new mutant populations of rice and evaluated its effectiveness by using a chemical mutagen diepoxybutane (DEB) with the expectation of manageably causing small-scale deletions that induce knock-out mutations. Compared with the more common mutagen, ethyl methanesulfonate (EMS), DEB exhibited approximately 160 times the adverse impact on the early growth of rice. At 0.3 mM, which was 1/160 the concentration of EMS in the control population, the heading date in the DEB-mutated population showed dispersion, albeit with a small standard deviation. Therefore, similar to EMS, DEB has been shown to induce DNA mutations. According to the screening of waxy mutants using the Targeting Induced Local Lesions in Genomes (TILLING) method, DEB-mutated populations had nearly similar mutation frequencies to those of EMS-mutated populations. Therefore, we successfully isolated five mutant lines from the DEB-mutated population. Some of these mutants exhibited a low-amylose phenotype, which is applicable to breeding leading to enhanced taste evaluation. To utilize these mutated alleles, we developed co-dominant DNA markers. In this study, EMS induced transition mutations, as previously reported. In contrast, DEB specifically induced transversion mutations rather than small-scale deletions contrary to our initial expectations. These results demonstrate that DEB has a novel point of action for mutation and is useful for expanding genetic resources for crops.
- Published
- 2020
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