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Start Over Author "Mazzarelli, G" Topic aids serodiagnosis

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1. Multicenter evaluation of the new HIV DUO assay for simultaneous detection of HIV antibodies and p24 antigen.

Author

Portincasa P, Grillo R, Pauri P, Colao MG, Valcavi PP, Speziale D, Mazzarelli G, De Majo E, Varaldo PE, Fadda G, Chezzi C, and Dettori G

Subjects
Blotting, Western, Evaluation Studies as Topic, HIV Seropositivity diagnosis, Humans, AIDS Serodiagnosis methods, Enzyme-Linked Immunosorbent Assay methods, HIV Antibodies blood, HIV Core Protein p24 blood
Abstract

A multicenter survey was performed to evaluate a new semi-automated human immunodeficiency virus fourth generation antibodies and antigen simultaneous assay. This assay showed a sensitivity of 100% and specificity of 99.6% among sera obtained from hospitalized patients or blood donors. Sera obtained from commercially available as well as in-house seroconversions were tested showing that HIV DUO is able to reveal an infected state in 11 out of 14 cases earlier than conventional tests. This new assay improves old test performances in terms of sensitivity, maintaining specificity at very high levels.

Published
2000

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