1. Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells
- Author
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Chen, Tianpeng, Hao, Jianxiong, He, Jinfeng, Zhang, Jianchun, Li, Yingcong, Liu, Rui, and Li, Lite
- Subjects
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AUTOPHAGY , *CELL death , *ANTINEOPLASTIC agents , *MTOR protein , *CELL cycle , *APOPTOSIS , *CANNABIS (Genus) , *PROTEIN kinase B , *LIVER cancer , *CELL lines , *CANCER cells - Abstract
Abstract: This study investigates the anticancer properties of cannabisin B, purified from hempseed hull, in HepG2 human hepatoblastoma cells. The results indicate that cannabisin B significantly inhibited cell proliferation by inducing autophagic cell death rather than typical apoptosis. Cell viability transiently increased upon the addition of a low concentration of cannabisin B but decreased upon the addition of high concentrations. Cannabisin B-induced changes in cell viability were completely inhibited by pre-treatment with 3-methyladenine (3-MA), indicating that the induction of autophagy by cannabisin B caused cell death. Additionally, cannabisin B induced S phase cell cycle arrest in a dose-dependent manner. Moreover, cannabisin B was found to inhibit survival signaling by blocking the activation of AKT and down-stream targets of the mammalian target of rapamycin (mTOR). These findings suggest that cannabisin B possesses considerable antiproliferative activity and that it may be utilised as a promising chemopreventive agent against hepatoblastoma disease. [Copyright &y& Elsevier]
- Published
- 2013
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