1. Phenylalanine inhibition of the phosphorylation of cytoskeletal proteins from cerebral cortex of young rats is prevented by alanine.
- Author
-
Carreras AL, de Mattos-Dutra A, Meirelles R, da Rocha BB, Wannmacher CM, and Pessoa-Pureur R
- Subjects
- Age Factors, Animals, Cerebral Cortex chemistry, Cytoskeletal Proteins analysis, Electrophoresis, Polyacrylamide Gel, Neurofilament Proteins metabolism, Phenylketonurias metabolism, Phosphorus Radioisotopes, Phosphorylation, Rats, Rats, Wistar, Tubulin metabolism, Alanine pharmacology, Cerebral Cortex metabolism, Cytoskeletal Proteins metabolism, Phenylalanine pharmacology
- Abstract
Background: Phenylalanine has been considered the main responsible agent for the brain damage that occurs in phenylketonuria., Methods and Results: In this work we studied the effect of this amino acid on the in vitro phosphorylation of cytoskeletal proteins of the cerebral cortex of rats. We observed that 2 mM phenylalanine, a concentration usually found in the plasma of phenylketonuric patients, decreased the in vitro 32P incorporation into these proteins. In addition, we investigated the effect of alanine on the inhibition of 32P incorporation into cytoskeletal proteins caused by phenylalanine. We observed that 0.5 mM alanine did not alter 32P incorporation but prevented the inhibition provoked by phenylalanine., Conclusion: In case the inhibition of cytoskeletal protein phosphorylation by phenylalanine also occurs in human phenylketonuria, it is possible that alanine supplementation to the phenylalanine-restricted diet may be beneficial to these patients.
- Published
- 2000
- Full Text
- View/download PDF