1. Virus inactivation in albumin by a combination of alkali conditions and high temperature.
- Author
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Roberts PL, More J, Rott J, and Lewin D
- Subjects
- Albumins chemistry, Albumins metabolism, Animals, Bocavirus drug effects, Bocavirus physiology, Cells, Cultured, Chlorocebus aethiops, Hepatitis A virus drug effects, Hepatitis A virus physiology, Humans, Hydrogen-Ion Concentration, Parvovirus, Canine drug effects, Parvovirus, Canine physiology, Protein Stability drug effects, Simian virus 40 drug effects, Simian virus 40 physiology, Vero Cells, Albumins pharmacology, Alkalies pharmacology, Hot Temperature, Virus Inactivation drug effects
- Abstract
Non-enveloped viruses such as HAV and B19 are of potential concern in plasma products. In the case of albumin, pasteurisation at 60 °C for 10 h is generally used for virus inactivation. However this procedure is only partially effective against some non-enveloped viruses. Using a range of non-enveloped viruses i.e. HAV, SV40, CPV, treatment at a high pH of about 9.5 and a temperature of 60 °C for 10 h was found to be effective for virus inactivation. These extreme conditions caused no increase in aggregate composition of the albumin. In addition the albumin composition was stable over a period of at least 6 months. The ligand binding properties of the albumin, as determined using the dye phenol red, were also not affected by this treatment. This procedure has the potential for increasing the spectrum of viruses inactivated by the 60 °C pasteurisation step., (Copyright © 2011 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2011
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