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In the present work, silver nanoparticles have been biosynthesized by utilizing the alcoholic extract ofPlumbago auriculata. The optimization of reaction conditions was carried out by monitoring the reactions with the help of UV–Visible absorption spectroscopy. The characterization of AgNP was carried out by infrared spectroscopy, transmission electron microscopy and X-Ray diffraction (XRD) studies. The biogenic AgNPs were tested againstMycobacterium tuberculosisusing Microplate Almar Blue assay (MABA) and their antioxidant activity was also evaluated. The silver nanoparticles were also assessed for their reducing activity against organic dyes. The AgNPs were spherical in shape with size ranging from 15 to 45 nm with face centered cubic geometry as revealed by XRD analysis. The AgNPs possessed good antitubercular activity with MIC value of 1.6 μg/ml and these also exhibited promising antioxidant activity with IC50value of 28.2. Furthermore, AgNPs also reduced congo red within 2 h and malachite green was degraded within 40 min. The present work demonstrated the utilization ofP. auriculatafor biosynthesis of AgNP which could be a potential candidate for antitubercular drug development and it could also be used as an antioxidant agent. The application of AgNP in reducing agent can be further extended and evaluated for purification of effluent water from textile industries. [ABSTRACT FROM AUTHOR]