1. Single step isolation and activation of primary CD3+ T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers.
- Author
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Kim K, An HJ, Jun SH, Kim TJ, Lim SA, Park G, Na HB, Park YI, Hyeon T, Yee C, Bluestone JA, Kim J, and Lee KM
- Subjects
- Animals, Antibodies, Monoclonal immunology, Antibodies, Monoclonal metabolism, CD3 Complex immunology, Mice, Mice, Inbred C57BL, T-Lymphocytes immunology, Alcohols, CD3 Complex metabolism, Cell Separation methods, Lymphocyte Activation, Magnetite Nanoparticles chemistry, Nanofibers chemistry, T-Lymphocytes cytology, T-Lymphocytes metabolism
- Abstract
Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3(+) T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8(+)CD3(+) T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.
- Published
- 2012
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