1. Laboratory culture for 14C-labeling for Chlorella and Oedogonium
- Author
-
George H. Wagner and Anna M. Krzywicka
- Subjects
Radiation ,Chromatography ,biology ,Chlorophyta ,Chlorella ,biology.organism_classification ,Plant cell ,Microbiology ,chemistry.chemical_compound ,Nuclear Energy and Engineering ,chemistry ,Algae ,Cell culture ,Isotope Labeling ,Carbon dioxide ,Oedogonium ,Radiology, Nuclear Medicine and imaging ,Carbon-14 ,Carbon Radioisotopes - Abstract
Algae were cultured in experiments that attained efficient CO 2 utilization permitting 14 C- labeling of cells and that compared growth characteristics of unicellular Chlorella sp. and filamentous Oedogonium sp . Culture vessels were 500 ml glass tubes through which air enriched to 5% CO 2 was slowly metered. The tubes, used in a vertical position for growing Chlorella , were filled with culture medium and the cells kept in suspension using a magnetic stirrer. Tubes placed horizontally and half filled with medium were used for Oedogonium permitting the algae to grow at the liquid-air interface. Maximum yield was 3 g/l. in 5 days for Chlorella and 1 g/0·5 l. in 10 days for Oedogonium . Efficiency and rate of CO 2 fixation, cell size and cell weight for the two algae are evaluated.
- Published
- 1975