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Multicomponent reactive transport modeling is a powerful tool for the comprehensive analysis of coupled hydraulic and biochemical processes. The performance of the simulation model depends on the accuracy of related model parameters whose values are usually difficult to determine from direct measurements. In this situation, estimates of these uncertain parameters can be obtained by solving inverse problems. In this study, an efficient data assimilation method, the iterative local updating ensemble smoother (ILUES), is employed for the joint estimation of hydraulic parameters, biochemical parameters and contaminant source characteristics in the sequential biodegradation process of tetrachloroethene (PCE). In the framework of the ILUES algorithm, parameter estimation is realized by updating local ensemble with the iterative ensemble smoother (IES). To better explore the parameter space, the original ILUES algorithm is modified by determining the local ensemble partly with a linear ranking selection scheme. Numerical case studies based on the sequential biodegradation of PCE are then used to evaluate the performance of the ILUES algorithm. The results show that the ILUES algorithm is able to achieve an accurate joint estimation of related model parameters in the reactive transport model. [ABSTRACT FROM AUTHOR]