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Start Over Author "Wang, Xiumin" Topic alkaline phosphatase
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1. Rapid cloning, expression and purification of a novel high-activity alkaline phosphatase with detoxification of lipopolysaccharide.

Author

Wu, Daichao, Teng, Da, Xi, Di, Wang, Xiumin, Wang, Xiaojie, Mao, Ruoyu, Zhang, Yong, Dai, Hua, and Wang, Jianhua

Subjects
*MOLECULAR cloning, *GENE expression, *ALKALINE phosphatase, *METABOLIC detoxification, *LIPOPOLYSACCHARIDES, *SACCHAROMYCES, *PICHIA pastoris
Abstract

Highlights: [•] First report of cloning of a novel AP gene from Saccharomyces boulardii. [•] Heterologous expression of high-activity rAP in Pichia pastoris X-33. [•] rAP possess favorable enzymatic characters. [•] rAP possess the function of dephosphorylation of LPS. [•] rAP alleviate inflammation responses caused by LPS. [Copyright &y& Elsevier]

Published
2014
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