1. Increased prevalence of celiac disease without gastrointestinal symptoms in adults MICA 5.1 homozygous subjects from the Campania area
- Author
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D Gennarelli, E Farinaro, Carolina Ciacci, A Spampanato, Lucia Sacchetti, Raffaella Tortora, Nadia Tinto, Giuseppe Calcagno, Tinto, N, Ciacci, C, Calcagno, G, Gennarelli, D, Spampanato, A, Farinaro, Eduardo, Tortora, R, Sacchetti, L., Tinto, Nadia, Ciacci, Carolina, Calcagno, Giuseppe, Gennarelli, Daniela, Tortora, Raffaella, and Sacchetti, Lucia
- Subjects
Adult ,Male ,Immunoglobulin A ,Genotype ,Human leukocyte antigen ,Major histocompatibility complex ,Coeliac disease ,HLA-DQ Antigens ,HLA-DQ ,Prevalence ,medicine ,Humans ,Genetic Predisposition to Disease ,Allele ,Polymorphism, Genetic ,Hepatology ,biology ,business.industry ,Histocompatibility Antigens Class I ,Gastroenterology ,HLA-DR Antigens ,medicine.disease ,Celiac Disease ,Italy ,Immunology ,biology.protein ,Female ,Antibody ,business - Abstract
OBJECTIVES: Polymorphisms in the major histocompatibility complex class I chain-related gene A may influence its binding to the Natural Killer Cell Receptor G2D (NKG2D). We looked for polymorphisms in major histocompatibility complex class I chain-related gene A exon 5 and in Human Leukocyte Antigen (HLA)-DQ/DR in adult coeliac disease patients to determine whether they affected coeliac disease phenotypes. METHODS: Adult coeliac disease patients with (n=98) and without (n=93) gastrointestinal symptoms (gastrointestinal symptoms+/gastrointestinal symptoms-) and 108 control subjects from Campania (Italy) were characterized by Polymerase Chain Reaction (PCR) sequence specific oligonucleotide followed by PCR sequence specific primer assays for HLA DQ/DR, and by PCR followed by capillary electrophoresis for major histocompatibility complex class I chain-related gene A exon 5 polymorphisms. Immunoglobulin A (IgA) anti-transglutaminase antibodies were also evaluated by immunosorbent assay. RESULTS: Five different major histocompatibility complex class I chain-related gene A alleles were detected in both coeliac disease patients and control subjects. The major histocompatibility complex class I chain-related gene A 5.1 allele occurred more frequently in patients than in controls (p
- Published
- 2008
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