Search

Your search keyword '"Fukai E"' showing total 2 results
Start Over Author "Fukai E" Topic alleles
2 results on '"Fukai E"'

1. Suppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele in Brassica self-incompatibility.

Author

Fujimoto R, Sugimura T, Fukai E, and Nishio T

Subjects
Amino Acid Sequence, Arabidopsis genetics, Arabidopsis metabolism, Cloning, Molecular, Flowers genetics, Flowers metabolism, Haplotypes, Heterozygote, Molecular Sequence Data, Plants, Genetically Modified, Reproduction genetics, Reproduction physiology, Transcription, Genetic genetics, Alleles, Brassica rapa genetics, Brassica rapa physiology, Gene Expression Regulation, Plant genetics, Genes, Plant genetics, Genes, Recessive genetics, Plant Proteins genetics
Abstract

Mutations in the S locus of a self-compatible cultivar Yellow Sarson in Brassica rapa, which has a self-compatible class-I S haplotype, S-f2, were investigated. S-28 in Brassica oleracea was found to be a member of an interspecific pair with S-f2 in B. rapa. The original S haplotype of S-f2 was identified to be S-54 in B. rapa. Sequence comparison of alleles in S-f2 with those in S-54 and B. oleracea S-28 revealed insertion of a retrotransposon-like sequence in the first intron of SRK and 89-bp deletion in the promoter region of SP11. No transcripts of SRK and SP11 were detected in S-f2 homozygotes, suggesting that the insertion and the deletion in SRK and SP11, respectively, caused the loss of the function of these genes. Promoter assay using transgenic plants indicated that the SP11 promoter of S-f2 has no activity. Heterozygotes of S-f2 and a normal class-II S haplotype, S-60, in B. rapa were found to be self-compatible. Interestingly, transcription of SP11-60 was revealed to be suppressed in the S-f2/S-60 heterozygotes, suggesting that an untranscribed class-I SP11 allele suppresses the expression of a recessive class-II SP11 allele in the anthers of S heterozygotes. Similar phenomenon was observed in heterozygotes of a self-compatible class-I S haplotype and a self-incompatible class-II S haplotype in B. oleracea.

Published
2006
Full Text
View/download PDF

2. Molecular genetic analysis of the candidate gene for MOD, a locus required for self-incompatibility in Brassica rapa

Author

Mikhail E. Nasrallah, Fukai E, and Takeshi Nishio

Subjects
Candidate gene, DNA Mutational Analysis, Restriction Mapping, Mutant, Locus (genetics), Brassica, Biology, Aquaporins, Ion Channels, Transformation, Genetic, Brassica rapa, Genetics, RNA, Messenger, Allele, Molecular Biology, Gene, Peptide sequence, Alleles, Plant Proteins, Models, Genetic, Homozygote, Nucleic acid sequence, Exons, Sequence Analysis, DNA, General Medicine, Oligonucleotides, Antisense, Blotting, Northern, Plants, Genetically Modified, Blotting, Southern, Gamma Rays, Mutation
Abstract

The MIP-MOD (for MOD-locus associated Major Intrinsic Protein) gene encodes an aquaporin-like product, and has been reported to be a candidate for the MOD gene which is required for the self-incompatibility response in Brassica rapa. In an antisense suppression experiment designed to investigate the role of MIP-MOD, we found that levels of MIP-MOD mRNA in the stigmas of fourteen antisense transgenics, as well as in the self-incompatible cultivar Osome (Osm), were much lower than in the stigmas of the self-incompatible S8 homozygous (S8) strain. Therefore, we analyzed the molecular structure of the MIP-MOD gene in three B. rapa strains: S8, Osm, and the self-compatible var. Yellow Sarson (YS). Nucleotide sequence analysis of the MIP-MOD genes isolated from the three strains revealed that all three encode the same amino acid sequence and that YS and Osm contain the same MIP-MOD allele, designated MIP-MOD(YS). Analysis of other self-incompatible B. rapa strains that are homozygous for the MIP-MOD(YS) allele indicated that high levels of MIP-MOD transcripts are not essential for the self-incompatibility response. Furthermore, a MOD mutant generated by gamma-irradiation was found to contain a wild-type MIP-MOD gene that is expressed at normal levels. These data suggest that MIP-MOD is not MOD itself. We suggest that this gene should be renamed MLM (for MIP gene linked to MOD).

Published
2001

Catalog

Books, media, physical & digital resources
See catalog results