24 results on '"HLA-B7 Antigen genetics"'
Search Results
2. Characterisation of the novel HLA-B*07:02:01:110 allele by next-generation sequencing.
- Author
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Kouniaki D, Athanassiades T, and Tsirogianni A
- Subjects
- Humans, HLA-B7 Antigen genetics, Exons, Sequence Analysis, DNA methods, Sequence Alignment, Alleles, High-Throughput Nucleotide Sequencing methods, 3' Untranslated Regions, Histocompatibility Testing methods, Base Sequence
- Abstract
HLA-B*07:02:01:110 differs from the HLA-B*07:02:01:01 allele by two nucleotide substitutions in the 3'UTR., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
3. HLA-B*13:64, a novel allele, identified in a Chinese individual.
- Author
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Li JP, Zhang X, Lin FQ, Zhang KL, and Li XF
- Subjects
- China, Codon, Genotype, HLA-A Antigens genetics, HLA-A11 Antigen genetics, HLA-B7 Antigen genetics, HLA-DRB1 Chains genetics, Hematopoietic Stem Cell Transplantation, Humans, Isoleucine genetics, Software, Threonine genetics, Alleles, HLA-B13 Antigen genetics
- Abstract
HLA-B*13:64 has one amino acid change from HLA-B*13:02:01:01 where 94 Threonine is changed to Isoleucine., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2019
- Full Text
- View/download PDF
4. Simple, rapid and inexpensive typing of common HLA class I alleles for immunological studies.
- Author
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Law SC, Haigh OL, Walpole CM, Keane C, Miles JJ, Gandhi MK, Radford KJ, and Steptoe RJ
- Subjects
- Humans, Alleles, DNA Primers genetics, HLA-A2 Antigen genetics, HLA-B7 Antigen genetics, HLA-B8 Antigen genetics, Histocompatibility Testing, Polymerase Chain Reaction
- Abstract
Current HLA-typing methods are typically designed to provide exquisitely-detailed identification of multiple HLA-alleles to satisfy the requirements for organ and bone marrow transplantation or genetic studies. Many human immunological studies, on the other hand, focus around only a small number of HLA alleles that are abundant or of relevance to specific diseases. Consequently, for such studies, many HLA typing approaches are not cost-effective and are potentially complicated, slow and not easily performed in-house. Work-flow would be streamlined by a simple, inexpensive and rapid typing method able to be performed in-house. We outline a straightforward approach that provides appropriate data for much immunological research. In a predominantly Caucasian population, flow cytometry using anti-HLA-A2, -B8 and -B7 antibodies consistently and accurately screened for samples carrying the highly-abundant HLA class I alleles HLA-A*02:01, -B*08:01 and -B*07:02 that form the focus of immunological studies. Next, we describe a straightforward and simple strategy for design and use of allele-specific PCR primers to identify, at high-resolution, alleles of interest. When combined with a simple gDNA extraction technique this provides reliable, simple and inexpensive in-house HLA typing demonstrated here for highly-abundant HLA class I alleles., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
5. Genomic sequences of HLA-A*68:169, HLA-B*07:298 and HLA-B*39:129.
- Author
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Balas A, Planelles D, Rodríguez-Cebriá M, Puig N, and Vicario JL
- Subjects
- Amino Acid Sequence, HLA-A Antigens chemistry, HLA-B Antigens chemistry, HLA-B7 Antigen chemistry, Haplotypes, Humans, Peptides chemistry, Alleles, Base Sequence, HLA-A Antigens genetics, HLA-B Antigens genetics, HLA-B7 Antigen genetics, Sequence Analysis, DNA
- Abstract
Three new HLA class I alleles were described in the Spanish population. HLA-A*68:169 and -B*39:129 show one amino acid replacement at the α1-domain, compared to A*68:02 (P47 > L47) and -B*39:06 (S11 > A11), respectively. HLA-B*07:298 presents one nucleotide mutation within exon 1, resulting in a new amino acid position -14, L>Q, which has not been previously described in any HLA protein. Prediction of the B*07:298 signal peptide cleavage did not show significant differences in comparison with that obtained for the rest of HLA-B genes., (© 2018 John Wiley & Sons Ltd.)
- Published
- 2018
- Full Text
- View/download PDF
6. Characterization of the novel HLA-B*07:305 allele by sequencing-based typing.
- Author
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Guidicelli G, Elsermans V, Top I, Ralazamahaleo M, and Visentin J
- Subjects
- Base Sequence, Exons genetics, Humans, Sequence Alignment, Alleles, HLA-B7 Antigen genetics, Histocompatibility Testing methods, Sequence Analysis, DNA
- Abstract
HLA-B*07:305 differs from HLA-B*07:02:01:01 by one nucleotide substitution at position 255., (© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2018
- Full Text
- View/download PDF
7. Discovery of a novel HLA-B*07 variant, HLA-B*07:294, in a Chinese individual.
- Author
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Liu B, Lv J, and Ma Z
- Subjects
- Amino Acid Substitution, Asian People, Base Sequence, Codon chemistry, Female, Gene Expression, Genotype, HLA-B7 Antigen immunology, Hematopoietic Stem Cell Transplantation, Histocompatibility Testing, Humans, Ovarian Neoplasms immunology, Ovarian Neoplasms pathology, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Transplant Recipients, Alleles, Exons, HLA-B7 Antigen genetics, Ovarian Neoplasms genetics, Polymorphism, Single Nucleotide
- Abstract
The novel HLA-B*07:294 allele differs from the closest matching allele, HLA-B*07:02:01 by 1 nucleotide substitution., (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
8. An HLA-B7-specific antibody in an HLA-B*07 positive patient explained by a nonexpressed allele (HLA-B*07:181N).
- Author
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Wenda S, Faé I, and Fischer GF
- Subjects
- Adult, Base Sequence, Female, Gene Expression, Genotype, HLA-B39 Antigen immunology, HLA-B7 Antigen immunology, High-Throughput Nucleotide Sequencing, Histocompatibility Testing, Humans, Polymerase Chain Reaction, Protein Isoforms genetics, Protein Isoforms immunology, Sequence Alignment, Sequence Analysis, DNA, Tissue Donors, Transplantation, Homologous, Alleles, HLA-B39 Antigen genetics, HLA-B7 Antigen genetics, Isoantibodies genetics, Kidney Transplantation methods, Transplant Recipients
- Abstract
Antibody identification by a bead array assay in a kidney patient revealed several HLA-specific antibodies including one directed against the HLA-B7 antigen. Low-resolution typing of the patient indicated the presence of an HLA-B*07 allele. To rule out an HLA-specific autoantibody the HLA-typing of the patient was further refined by nucleotide sequencing on a next-generation sequencing platform and eventually showed an HLA-B*39:01:01:03 and HLA-B*07:181N genotype. Thereby the allospecific nature of the antibody was proven. The HLA-B7-specific antibody could be explained by an immunization during the first kidney-transplantation in 1996 with an HLA-B*07 positive donor. When assessing the plausibility of antibodies, the presence of nonexpressed alleles should be taken into consideration., (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
9. Full-length sequences of 3 HLA-B alleles, B*07:05:01:01, B*14:01:01 and B*18:02, confirmed by cloning and sequencing.
- Author
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Li Z and Zou HY
- Subjects
- Asian People, Base Sequence, Cloning, Molecular, Codon chemistry, Gene Expression, HLA-B14 Antigen immunology, HLA-B18 Antigen immunology, HLA-B7 Antigen immunology, Hematopoietic Stem Cell Transplantation, Histocompatibility Testing, Humans, Introns, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Tissue Donors, Alleles, Exons, HLA-B14 Antigen genetics, HLA-B18 Antigen genetics, HLA-B7 Antigen genetics, Polymorphism, Genetic
- Abstract
Full-length sequences of HLA-B*07:05:01:01, B*14:01:01 and B*18:02, confirmed by cloning and sequencing in Chinese donors., (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
10. Identification of a novel HLA-B*07 variant, B*07:269, by sequence-based typing in a Taiwanese bone marrow stem cell donor.
- Author
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Yang KL and Lin PY
- Subjects
- Amino Acid Sequence, Amino Acid Substitution, Base Sequence, Codon chemistry, HLA-B7 Antigen immunology, Haplotypes, Hematopoietic Stem Cell Transplantation, Histocompatibility Testing, Humans, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Taiwan, Alleles, Exons, HLA-B7 Antigen genetics, Point Mutation, Tissue Donors
- Abstract
One nucleotide replacement at residue 559 of HLA-B*07:05:01:01 or B*07:06:01 results in a novel allele, HLA-B*07:269., (© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2016
- Full Text
- View/download PDF
11. Identification of the novel HLA-B*07:261 allele.
- Author
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Brunet L, Mouron D, Villard J, Tiercy JM, and Buhler S
- Subjects
- Humans, Alleles, HLA-B7 Antigen genetics
- Published
- 2016
- Full Text
- View/download PDF
12. Detection of a novel variant of HLA-B*07, HLA-B*07:249, in a Taiwanese unrelated hematopoietic stem cell donor.
- Author
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Yang KL, Hung JH, and Lin PY
- Subjects
- Base Sequence, Codon, Exons, HLA-B7 Antigen immunology, Hematopoietic Stem Cell Transplantation, Histocompatibility Testing, Humans, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Taiwan, Unrelated Donors, Alleles, HLA-B7 Antigen genetics, Point Mutation
- Abstract
One nucleotide replacement at residue 398 of HLA-B*07:05:01 or HLA-B*07:06 results in a novel allele, HLA-B*07:249., (© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2015
- Full Text
- View/download PDF
13. HLA-B27, but not HLA-B7, immunodominance to influenza is ERAP dependent.
- Author
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Akram A, Lin A, Gracey E, Streutker CJ, and Inman RD
- Subjects
- Aminopeptidases genetics, Animals, Gene Expression Regulation genetics, Gene Expression Regulation immunology, Genetic Predisposition to Disease, HLA-B27 Antigen genetics, HLA-B7 Antigen genetics, Humans, Influenza A Virus, H3N2 Subtype genetics, Mice, Orthomyxoviridae Infections genetics, Orthomyxoviridae Infections pathology, Spondylitis, Ankylosing genetics, Spondylitis, Ankylosing immunology, Spondylitis, Ankylosing pathology, Alleles, Aminopeptidases immunology, HLA-B27 Antigen immunology, HLA-B7 Antigen immunology, Influenza A Virus, H3N2 Subtype immunology, Orthomyxoviridae Infections immunology
- Abstract
Endoplasmic reticulum-associated aminopeptidase-1 (ERAP1) plays a critical role in the processing of peptides prior to binding to MHC class I molecules. In this article, we show for the first time, to our knowledge, that the HLA-B27 immunodominant influenza nucleoprotein (NP) 383-391 epitope is made as an N-terminally extended 14-mer before it is trimmed by ERAP. In the absence of ERAP, there is a significant reduction in the CTL response to the B27/NP383-391 epitope in influenza A (flu)-infected B27/ERAP(-/-) mice. With the use of tetramer staining, the number of naive CD8(+) T cells expressing TCR Vβ8.1 in B27/ERAP(-/-) transgenic mice is significantly lower than that seen in B27/ERAP(+/+) mice. HLA-B27 surface expression in naive and flu-infected B27/ERAP(-/-) mice is also lower than the expression seen for the same allele in naive and flu-infected B27/ERAP(+/+) mice. In contrast, surface expression of HLA-B7 was unaffected by the absence of ERAP in B7/ERAP(-/-) transgenic mice. The B7-restricted NP418-426 CTL response in flu-infected B7/ERAP(-/-) and B7/ERAP(+/+) mice was also similar. These results provide, to our knowledge, the first in vivo demonstration of ERAP functionally influencing host immune response in an HLA allele-specific manner. This principle has relevance to diseases such as ankylosing spondylitis, in which HLA-B27 and ERAP jointly contribute to disease predisposition., (Copyright © 2014 by The American Association of Immunologists, Inc.)
- Published
- 2014
- Full Text
- View/download PDF
14. Identification of a novel HLA-B allele, B*07:162, in a Taiwanese individual.
- Author
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Chen PL, Lai SK, Yang WS, Chang TC, and Chu CC
- Subjects
- Base Sequence, Exons genetics, Humans, Molecular Sequence Data, Sequence Alignment, Taiwan ethnology, Alleles, Asian People genetics, HLA-B7 Antigen genetics
- Abstract
The new allele, HLA-B*07:162, is identical to HLA-B*07:12 in exon 2 but has a non-synonymous substitution at position 419 (A to C) in exon 3., (© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2013
- Full Text
- View/download PDF
15. A novel HLA-B allele, B*07:55, identified by sequence-based typing.
- Author
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Liu N, Zhang Z, Shan X, Wang L, and Cui S
- Subjects
- Base Sequence, Exons genetics, Humans, Molecular Sequence Data, Sequence Alignment, Alleles, HLA-B7 Antigen genetics, Histocompatibility Testing
- Abstract
The novel allele differs from HLA-B*07:02:01 by a single nucleotide substitution at position 538 in exon 3., (© 2013 John Wiley & Sons A/S.)
- Published
- 2013
- Full Text
- View/download PDF
16. Genomic full-length analysis of the B*08:79 allele suggests exon shuffling involving the B*08:01:01 and B*07:06 alleles.
- Author
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Balas A, Planelles D, Solves P, Roig R, and Vicario JL
- Subjects
- Base Sequence, Humans, Molecular Sequence Data, Alleles, Exons genetics, Genome, Human genetics, HLA-B7 Antigen genetics, HLA-B8 Antigen genetics, Recombination, Genetic genetics
- Abstract
B*08:79 is composed by partial B*08:01:01 and B*07:06 sequences because of a possible recombination event within intron 2., (© 2012 John Wiley & Sons A/S.)
- Published
- 2012
- Full Text
- View/download PDF
17. Association of HLA loci alleles and antigens in Saudi patients with vitiligo.
- Author
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Abanmi A, Al Harthi F, Al Baqami R, Al Assaf S, Zouman A, Arfin M, and Tariq M
- Subjects
- Adolescent, Adult, Case-Control Studies, Female, Gene Frequency, HLA-B Antigens genetics, HLA-B15 Antigen, HLA-B7 Antigen genetics, HLA-C Antigens genetics, HLA-DR Antigens genetics, HLA-DRB1 Chains, Humans, Male, Middle Aged, Saudi Arabia, Vitiligo ethnology, Vitiligo genetics, Alleles, Histocompatibility Antigens Class I genetics, Histocompatibility Antigens Class II genetics, Vitiligo immunology
- Abstract
HLA complex is composed of several closely linked loci, each containing several alleles, yielding a high expression of polymorphism. Vitiligo, a commonly acquired dermatological disorder, has been associated with different HLA antigens in different ethnic groups. In this study, HLA classes I (HLA-A, B, and C) and II (HLA-DR, DQ) antigens/alleles were analyzed in a group of 80 Saudi subjects consisting of vitiligo patients (40) and matched controls (40). The frequency of antigens of various HLA loci was tested using two-stage microcytotoxicity assays, while the frequency of alleles of HLA-DR was screened by polymerase chain reaction/sequence specific primers (PCR/SSP) method. The frequencies of HLA-B7, B15, Bw6, Cw6, Cw7, and DRB4*010101 were found to be significantly higher in vitiligo patients compared to controls [P = 0.029, 0.015, 0.033, 0.009, 0.043, and 0.015, respectively, with relative risk (RR) > or = 3, etiologic fraction (EF) > or = 0.4]. On the other hand, HLA-A9, B5, DQ1, and DRB3*010101 were significantly decreased in vitiligo patients compared to healthy Saudis [P = 0.008, 0.004, 0.028, and 0.04, respectively, with RR < 1 and preventive fraction (PF) < 0.5]. Among the patients, the highest allele frequency was noted for DRB4*010101(70%), while in controls it was for DRB3*010101 (72.5%). These results for antigens and allele frequency of various HLA Loci in vitiligo patients and control subjects suggested that HLA-B7, Bw6, Cw6, Cw7, and DRB4*010101 could be susceptible to vitiligo, while HLA-A9, B5, DQ1, and DRB3*010101 might be negatively associated with the development of vitiligo in Saudis.
- Published
- 2006
- Full Text
- View/download PDF
18. The HLA-B7 allele confers susceptibility to breast cancer in Spanish women.
- Author
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Lavado R, Benavides M, Villar E, Ales I, Alonso A, and Caballero A
- Subjects
- Breast Neoplasms pathology, Female, Humans, Spain, Alleles, Breast Neoplasms genetics, Genetic Predisposition to Disease genetics, HLA-B7 Antigen genetics
- Abstract
Background: Several HLA alleles are associated with susceptibility or protection in breast cancer. The particular allele varies depending on the geographical region. A study in a small group of Spanish patients using serological methods found an association with HLA-B7. We undertook a larger study in southern Spain using molecular biology techniques., Methods: Genotype variants of HLA class I and II were typed by PCR-SSP in 132 breast cancer patients and 382 healthy controls., Results: The frequency of the HLA-B7 allele was increased in the patients compared to the controls (P=0.0019; 95% confidence interval, 1.337-3.409, relative risk=2.135). Bonferroni correction of the P showed it was still significant (P(c)=0.0285)., Conclusions: These results support previous suggestions that HLA-B7 is associated with the development of breast cancer in our area.
- Published
- 2005
- Full Text
- View/download PDF
19. Undifferentiated spondyloarthropathies in Brazilians: importance of HLA-B27 and the B7-CREG alleles in characterization and disease progression.
- Author
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Sampaio-Barros PD, Conde RA, Donadi EA, Kraemer MH, Persoli L, Coimbra IB, Costallat LT, Samara AM, and Bértolo MB
- Subjects
- Adolescent, Brazil epidemiology, Child, Disease Progression, Female, Humans, Infant, Joints pathology, Male, Outpatients, Prospective Studies, Spondylitis epidemiology, Spondylitis pathology, Alleles, Genetic Predisposition to Disease, HLA-B27 Antigen genetics, HLA-B7 Antigen genetics, Spondylitis genetics
- Abstract
Objective: To analyze the profile of the HLA-B27 and B7 cross-reactive group (CREG) alleles and the role of these markers in disease characterization and progression in patients with undifferentiated spondyloarthropathies (uSpA)., Methods: A total of 80 patients with a diagnosis of uSpA (40 HLA-B27 positive and 40 HLA-B27 negative) were prospectively studied for 2 years. The control group consisted of 66 HLA-B27 positive and 112 HLA-B27 negative individuals without a history of seronegative SpA. HLA-B alleles were typed at low (B7-CREG alleles, i.e., B*7, B*54, B*55, B*56, B*40, B*42) or high resolution (B*27 alleles) using polymerase chain reaction-amplified DNA hybridized with sequence-specific oligonucleotide probes., Results: HLA-B*2705 was the most frequent allele, observed in 92.5% of the patients and in 77% of the controls, followed by the HLA-B*2702, observed in 5% of the patients and in 12% of the controls. HLA-B*2704 was observed in only one patient (2.5%), and was absent in the control population. HLA-B*2703 (6%) and HLA-B*2707 (5%) alleles were observed only in controls. No associations between HLA-B*27 alleles or B7-CREG alleles and any specific manifestation of uSpA were observed. HLA-B27 positive patients more frequently presented juvenile onset SpA (p = 0.002) and progression to ankylosing spondylitis (AS) (p = 0.03) than did HLA-B27 negative patients. The B7-CREG alleles were observed in 5% of the HLA-B27 positive uSpA group, in 25% of the HLA-B27 negative uSpA group, in 7% of the HLA-B27 positive controls, and in 13% of the HLA-B27 negative controls; a significant association was observed between the presence of the B7-CREG and the HLA-B27 negative uSpA group (p = 0.012)., Conclusion: The frequency of the HLA-B*2705 allele among the B27 positive uSpA patients of this series was closely similar to that reported for patients with ankylosing spondylitis (AS). The presence of HLA-B*27 alleles was associated with the progression to AS, and the presence of B7-CREG was associated with uSpA in the HLA-B27 negative group.
- Published
- 2003
20. Association of HLA class I and class II alleles with susceptibility to endometriosis.
- Author
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Kitawaki J, Obayashi H, Kado N, Ishihara H, Koshiba H, Maruya E, Saji H, Ohta M, Hasegawa G, Nakamura N, Yoshikawa T, and Honjo H
- Subjects
- Adult, Endometriosis genetics, Female, Gene Frequency, HLA-A Antigens genetics, HLA-A24 Antigen, HLA-B7 Antigen genetics, HLA-DR Antigens genetics, HLA-DRB1 Chains, Haplotypes, Humans, Linkage Disequilibrium, Middle Aged, Alleles, Endometriosis immunology, Genes, MHC Class I, Genes, MHC Class II, Genetic Predisposition to Disease
- Abstract
Although the exact etiology of endometriosis is unclear, several lines of evidence support roles for both cell-mediated and humoral immunity in its pathogenesis. To assess the association between HLA genotypes and endometriosis, we investigated the frequencies of HLA-A, -B, -C, and -DRB1 antigens or alleles in 123 Japanese patients with endometriosis and 165 healthy women as controls. Significant positive association with endometriosis was observed for HLA-B7 (OR = 2.7, 95% CI = 1.5-5.1, p(u) = 0.0022, p(c) = 0.0440) and for Cw*0702 (OR = 2.1, 95% CI = 1.2-3.3, p(u) = 0.0026, p(c) = 0.0398). An increased frequency of DRB1*0101 was observed in endometriosis patients compared with control subjects (OR = 2.3, 95% CI = 1.2-4.4, p(u) = 0.0143), but was not statistically significant after correction for multiple comparisons. Two-locus analysis indicated that the susceptibility to endometriosis was primarily associated with B7, and that the increased frequencies of Cw*0702 and DRB1*0101 in patients reflected the linkage disequilibrium between B7 and Cw*0702 and DRB1*0101. Most of the B7 antigens were encoded by the B*0702 allele, which was in complete linkage disequilibrium with A24, Cw*0702, and DRB1*0101. Therefore, our results indicated that the HLA-A24-B*0702-Cw*0702-DRB1*0101 haplotype was associated with endometriosis susceptibility. Our findings may provide an important clue to elucidating the pathogenesis of endometriosis.
- Published
- 2002
- Full Text
- View/download PDF
21. Characterisation of a new HLA-B allele, HLA-B*0724.
- Author
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Middleton D, Curran MD, Anholts JD, Reilly ER, and Schreuder GM
- Subjects
- Base Sequence, Female, HLA-B7 Antigen chemistry, HLA-B8 Antigen chemistry, HLA-B8 Antigen genetics, Humans, Molecular Sequence Data, Alleles, HLA-B7 Antigen genetics
- Published
- 2001
- Full Text
- View/download PDF
22. Increased diversity within the HLA-B*07 group: identification of the two novel alleles B*0709 and B*0710.
- Author
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Elsner HA and Blasczyk R
- Subjects
- Amino Acid Sequence, Genetic Variation, Humans, Male, Molecular Sequence Data, Alleles, HLA-B7 Antigen genetics
- Abstract
The identification of the two novel alleles, HLA-B*0709 and B*0710, is described. B*0709 differs from B*07021 by a nucleotide exchange at position 419 (A>C) which is located in exon 3. At the protein level the nucleotide exchange results in an amino acid residue difference (Tyr116Ser). The other newly detected allele, B*0710, differs from B*07021 by a nucleotide exchange at position 272 (A>G) which is located in exon 2. This nucleotide exchange also leads to an amino acid substitution (Tyr67Cys). The allogeneic potential in case of mismatch with other alleles of the B*07 group at bone marrow transplantation was assessed. The peptide motifs between B*0709 and B*0711 may be very similar and thus the alloreactive potential in case of mismatch may be low. In contrast, mismatches of B*0709 and B*0710 with other B*07 alleles are likely to stimulate alloreactive T cells.
- Published
- 2000
- Full Text
- View/download PDF
23. CD8+ T cells are necessary for recognition of allelic, but not locus-mismatched or xeno-, HLA class I transplantation antigens.
- Author
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Borenstein SH, Graham J, Zhang XL, and Chamberlain JW
- Subjects
- Animals, B-Lymphocytes immunology, B-Lymphocytes metabolism, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes metabolism, Cytotoxicity Tests, Immunologic, Gene Expression Regulation immunology, Genetic Markers immunology, Graft Rejection genetics, Graft Rejection immunology, H-2 Antigens biosynthesis, H-2 Antigens genetics, HLA Antigens biosynthesis, HLA-A2 Antigen biosynthesis, HLA-A2 Antigen genetics, HLA-B27 Antigen biosynthesis, HLA-B27 Antigen genetics, HLA-B7 Antigen biosynthesis, HLA-B7 Antigen genetics, Histocompatibility Antigens Class I biosynthesis, Humans, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, Mice, Knockout, Mice, Transgenic, Skin Transplantation immunology, Transgenes immunology, Tumor Cells, Cultured, Alleles, Antigens, Heterophile genetics, CD8-Positive T-Lymphocytes immunology, HLA Antigens genetics, Histocompatibility Antigens Class I genetics, Histocompatibility Testing, Lymphocyte Activation genetics
- Abstract
Although HLA transgenic mice (HLA TgM) could provide a powerful approach to investigate human MHC-specific T cell responsiveness, the extent to which these molecules are recognized by the mouse immune system remains unclear. We established TgM expressing HLA class I alleles A2, B7, or B27 in their fully native form (HLAnat) or as hybrid molecules (HLAhyb) of the HLA alpha1/alpha2 domains linked to the H-2Kb alpha3, transmembrane, and cytoplasmic domains (i.e., to maintain possible species-specific interactions). Comparison of each as xeno- (i.e., by non-TgM) vs allo- (i.e., by TgM carrying an alternate HLA allele) transplantation Ags revealed the following: 1) Although HLAhyb molecules induced stronger xeno-CD8+ T cell responses in vitro, additional effector mechanisms must be active in vivo because HLAnat skin grafts were rejected faster by non-TgM; 2) gene knockout recipients showed that xenorejection of HLAnat and, unexpectedly, HLAhyb grafts doesn't depend on CD8+ or CD4+ T cells or B cells; 3) each HLAhyb strain developed tolerance to "self" but rejected allele- (-B27 vs -B7) and locus- (-B vs -A) mismatched grafts, the former requiring CD8+ T cells, the latter by CD8+ T cell-independent mechanisms. The finding that recognition of xeno-HLAhyb does not require CD8+ T cells while recognition of the identical molecule in a strictly allo context does, demonstrates an alpha1/alpha2 domain-dependent difference in effector mechanism(s). Furthermore, the CD8+ T cell-independence of locus-mismatched rejection suggests the degree of similarity between self and non-self alpha1/alpha2 determines the effector mechanism(s) activated. The HLA Tg model provides a unique approach to characterize these mechanisms and develop tolerance protocols in the context of human transplantation Ags.
- Published
- 2000
- Full Text
- View/download PDF
24. Sequence of a new HLA-B7 variant, B*0707, that differs from the common B*0702 allele by one single residue in the peptide binding groove.
- Author
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Grundschober C, Rufer N, Adami N, Breur-Vriesendorp B, Jeannet M, Roosnek E, and Tiercy JM
- Subjects
- Amino Acid Sequence, Base Sequence, Binding Sites genetics, HLA-B7 Antigen immunology, Humans, Molecular Sequence Data, Peptides immunology, Sequence Analysis, Alleles, HLA-B7 Antigen genetics
- Published
- 1997
- Full Text
- View/download PDF
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