1. HLA-DQB1 Allele Polymorphism Associated with Oral Submucous Fibrosis in Hunan, China.
- Author
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Tan Y, Huang Y, Guo L, Zhou L, Zhu K, Li Y, and Tan J
- Subjects
- Humans, Male, Female, China epidemiology, Adult, Middle Aged, Genotype, Case-Control Studies, Young Adult, Genetic Association Studies, HLA-DQ beta-Chains genetics, Genetic Predisposition to Disease, Alleles, Gene Frequency, Polymorphism, Genetic, Oral Submucous Fibrosis genetics
- Abstract
Methods: 44 OSF patients and 44 healthy volunteers were included in this study. To detect the expression frequency of HLA-DQB1 alleles in the two groups and analyze significant allelic subtypes and their relative risk, polymerase chain reaction (PCR) sequence-specific primers were used. Subsequently, based on the identification of differential genes, we compare the gene expression levels of OSF patients and healthy volunteers expressing differential genes by real-time quantitative PCR., Results: The expression frequency of the HLA-DQB1
∗ 05 : 02 allele in the OSF group (36.4%) was significantly higher than in the controls (13.6%), and exposure to the HLA-DQB1∗ 05 : 02 allele was strongly related to OSF (OR (95% CI) = 3.619 (1.257,10.421), Wald χ2 = 5.681, P =0.017). However, there were no significant differences in the allele expression frequencies of DQB1∗ 02 : 01, DQB1∗ 03 : 03, DQB1∗ 05 : 01, DQB1∗ 05 : 03, DQB1∗ 06 : 02, DQB1∗ 06 : 03, and DQB1∗ 06 : 04 in the OSF group compared with the controls (all P > 0.05). Furthermore, the relative expression level of the HLA-DQB1∗ 05 : 02 allele in the OSF group (3.98 ± 3.50) was significantly higher than in controls (0.70 ± 0.41)., Conclusions: There are differences in the HLA-DQB1 allele polymorphisms between the healthy population and patients with oral submucosal fibrosis. Preliminarily, it is suggested that the HLA-DQB1∗ 05 : 02 allele, which has a strong correlation with OSF and great differential expression between patients with OSF and controls, might be a susceptibility gene for OSF in Hunan., Competing Interests: The authors have no conflicts of interest to declare., (Copyright © 2024 Yisi Tan et al.)- Published
- 2024
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