1. Inhibitory Effect of Eriodictyol on IgE/Ag-Induced Type I Hypersensitivity
- Author
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Jung-Min Yoo, Yeo-Jin Kang, Sae-Jin Park, Jihee Kim, and Tack-Joong Kim
- Subjects
Hypersensitivity, Immediate ,Ceramide ,Gene Expression ,Acer ,Pharmacology ,medicine.disease_cause ,Immunoglobulin E ,Applied Microbiology and Biotechnology ,Biochemistry ,Cell Degranulation ,Analytical Chemistry ,Mice ,chemistry.chemical_compound ,Ceramide kinase ,Anti-Allergic Agents ,medicine ,Animals ,Mast Cells ,RNA, Messenger ,Molecular Biology ,Cells, Cultured ,Dose-Response Relationship, Drug ,biology ,Passive Cutaneous Anaphylaxis ,Organic Chemistry ,Degranulation ,General Medicine ,Eriodictyol ,medicine.disease ,Eriodictyon ,beta-N-Acetylhexosaminidases ,Rats ,Phosphotransferases (Alcohol Group Acceptor) ,chemistry ,Flavanones ,Allergic response ,Immunology ,biology.protein ,Cytokines ,Calcium ,Biomarkers ,Histamine ,Biotechnology ,Type I hypersensitivity - Abstract
Mast cells are the principal effector cells involved in the allergic response, through the release of histamine. We investigated the effect of eriodictyol, derived from the painted maple and yerba santa, on mast cell degranulation and on an allergic response in an animal model. We also investigated its effect on the expression of the ceramide kinase (CERK) involved in calcium-dependent degranulation, and on ceramide activation by multiple cytokines. Eriodictyol suppressed the release of beta-hexosaminidase, a marker of degranulation, and the expression of interleukin (IL)-4 mRNA. It inhibited the expression of CERK mRNA, reduced the ceramide concentration in antigen-stimulated mast cells, and suppressed the passive cutaneous anaphylaxis (PCA) reaction in mice in a dose-dependent manner. These results suggest that eriodictyol can inhibit mast cell degranulation through inhibition of ceramide kinase, and that it might potentially serve as an anti-allergic agent.
- Published
- 2012
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