1. A recombinant fragment of human SP-D reduces allergic responses in mice sensitized to house dust mite allergens.
- Author
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Strong, P., Townsend, P., Mackay, R., Reid, K.B.M., and Clark, H.W.
- Subjects
ALLERGIES ,HOUSE dust mites ,ALLERGENS - Abstract
C57Bl6 mice sensitized to Dermatophagoides pteronyssinus and challenged with D. pteronyssinus allergen extract given intranasally followed by treatment with intranasal applications of a 60-kDa truncated, trimeric recombinant form of human SP-D (rfhSP-D) showed a significant reduction in serum IgE, IgG1, peripheral blood eosinophilia and airway hyperresponsiveness compared to saline or bovine serum albumin-treated controls. Intracellular cytokine staining of lung and spleen homogenates showed increases in interleukin (IL)-12 production in lung tissue and normalization of IL-12 and interferon (IFN)- γ in spleen tissue. In previous studies we demonstrated the effectiveness of native SP-D and rfhSP-D in down-regulating allergic responses to allergens of Aspergillus fumigatus. The results reported here indicate that rfhSP-D can suppress the development of allergic symptoms in sensitized mice challenged with allergens of the common house dust mite. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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