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Your search keyword '"Leusink-Muis T"' showing total 3 results
Start Over Author "Leusink-Muis T" Topic allergy
3 results on '"Leusink-Muis T"'

2. The development of allergic inflammation in a murine house dust mite asthma model is suppressed by synbiotic mixtures of non-digestible oligosaccharides and Bifidobacterium breve M-16V

Author

Verheijden, K A T, Willemsen, L E M, Braber, S, Leusink-Muis, T, Jeurink, P V, Garssen, J, Kraneveld, A D, Folkerts, G, Pharmacology, Sub Immunopharmacology, LS Pharma, Sub Airway in vivo Pharmacology, Sub General Pharmacology, Pharmacology, Sub Immunopharmacology, LS Pharma, Sub Airway in vivo Pharmacology, and Sub General Pharmacology

Subjects
0301 basic medicine, Male, Allergy, Synbiotics, ved/biology.organism_classification_rank.species, Medicine (miscellaneous), Oligosaccharides, Bifidobacterium breve, Mice, 0302 clinical medicine, immune system diseases, Lung, Mice, Inbred BALB C, Nutrition and Dietetics, biology, Pyroglyphidae, Original Contribution, respiratory system, medicine.symptom, Bronchoalveolar Lavage Fluid, Inflammation, Allergic inflammation, House dust mite, 03 medical and health sciences, Interferon-gamma, medicine, Hypersensitivity, Animals, Asthma, Chemokine CCL22, ved/biology, business.industry, Interleukins, biology.organism_classification, medicine.disease, respiratory tract diseases, Eosinophils, Disease Models, Animal, 030104 developmental biology, Immunology, Chemokine CCL17, business, 030215 immunology
Abstract

PURPOSE: The incidence and severity of allergic asthma is rising, and novel strategies to prevent or treat this disease are needed. This study investigated the effects of different mixtures of non-digestible oligosaccharides combined with Bifidobacterium breve M-16V (BB) on the development of allergic airway inflammation in an animal model for house dust mite (HDM)-induced allergic asthma. METHODS: BALB/c mice were sensitized intranasally (i.n.) with HDM and subsequently challenged (i.n.) with PBS or HDM while being fed diets containing different oligosaccharide mixtures in combination with BB or an isocaloric identical control diet. Bronchoalveolar lavage fluid (BALF) inflammatory cell influx, chemokine and cytokine concentrations in lung homogenates and supernatants of ex vivo HDM-restimulated lung cells were analyzed. RESULTS: The HDM-induced influx of eosinophils and lymphocytes was reduced by the diet containing the short-chain and long-chain fructo-oligosaccharides and BB (FFBB). In addition to the HDM-induced cell influx, concentrations of IL-33, CCL17, CCL22, IL-6, IL-13 and IL-5 were increased in supernatants of lung homogenates or BALF and IL-4, IFN-γ and IL-10 were increased in restimulated lung cell suspensions of HDM-allergic mice. The diet containing FFBB reduced IL-6, IFN-γ, IL-4 and IL-10 concentrations, whereas the combination of galacto-oligosaccharides and long-chain fructo-oligosaccharides with BB was less potent in this model. CONCLUSION: These findings show that synbiotic dietary supplementation can affect respiratory allergic inflammation induced by HDM. The combination of FFBB was most effective in the prevention of HDM-induced airway inflammation in mice.

Published
2015

3. Antibodies directed against nerve growth factor inhibit the acute bronchoconstriction due to allergen challenge in guinea-pigs.

Author

De Vries, A, Engels, F, Henricks, P. A. J, Leusink-Muis, T, Fischer, A, and Nijkamp, F. P

Subjects
NERVE growth factor, BRONCHIAL spasm, PROTEIN-tyrosine kinases
Abstract

Background We have previously demonstrated that the administration of nerve growth factor (NGF) to guinea-pigs results in airway hyper-responsiveness within 1 h. Objective In the present study we document the involvement of NGF in the acute allergic airway response. Methods Guinea-pigs that are sensitized to ovalbumin show an acute bronchoconstriction directly after challenge with ovalbumin. Results Intratracheal application of 10 µg of antibodies directed against NGF (anti-NGF) 1 h before the challenge reduces the acute severe bronchoconstriction to approximately 40% and the sustained bronchoconstriction to approximately 20% of the reaction in controls. This shows a high potency of anti-NGF in diminishing the direct bronchoconstriction. Inhibition of the tyrosine kinases of the tyrosine kinase receptor A, the high-affinity receptor for NGF, has no effect on the bronchoconstriction. Therefore, we postulate that the p75, the low-affinity receptor for neurotrophins, is responsible for the acute bronchoconstriction. Our findings suggest a role for NGF in the induction of the acute asthmatic reaction. Conclusion These findings offer a new potential therapeutic strategy for the treatment of allergic asthma. [ABSTRACT FROM AUTHOR]

Published
2002
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