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The paper-based device for allopurinol detection was developed by an enzymatic reaction. The concept is that allopurinol will act by blocking the xanthine oxidase enzyme to produce uric acid and H2O2, resulting in a color change from purple to pale purple until it is colorless. The concept was proofed by spectrophotometry and was applied in four types of filter paper including Whatman Qualitative No. 1, No. 4, and No. 6, and Whatman 1 chromatography. The application of this system was observed using ImageJ analysis. The performance of the paper reveals that each Whatman has different characteristics with LOD and LOQ range were 1.62-2.24 ppm and 4.91-6.80 ppm, respectively. The stability study shows that all of the paper was stable for 3 days both at room temperature (around 24°C-26°C) and 4°C. The presence of another drug like paracetamol did not prevent allopurinol detection. The real traditional medicine sample was also analyzed to confirm the applicability of this paper, and the result showed good agreement with spectrophotometry data. [ABSTRACT FROM AUTHOR]

Traditional herbal medicine in Indonesia is still in great demand and popular in society. The Indonesian government regulations state that herbal medicine should not contain chemical drug due to the toxic effect of uncontrolled consumption. Allopurinol is one of the drugs commonly added to herbal medicine for the treatment of chronic gout. Paper-based analytical device is one of the latest forms of analysis that has been widely used for the identification of chemical elements, environmental contamination, bacteria, and many more. In this study, experiments were conducted using Whatman filter paper No. 1, No. 2, and No. 4 and Whatman chromatography as a paper, and 9 colorimetric reagents were tested for allopurinol detection in herbal medicine. There were 5 specific reagents that reacted positively with allopurinol and only 3 reagents that can be applied to the paper, that is, Folin-Ciocalteu, Tollens, and p-DAB reagent. The results of the optimization show that the most optimal immersion time was 60 minutes with a drying time of 30 minutes at 50°C. Each filter paper has different characteristic; however, there was no significant difference when all of the papers were used as PAD for allopurinol detection. [ABSTRACT FROM AUTHOR]