1. Genotyping of Toxoplasma gondii in a lethal toxoplasmosis outbreak affecting captive howler monkeys (Alouatta sp.).
- Author
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Santana CH, de Oliveira AR, Dos Santos DO, Pimentel SP, de Souza LDR, Moreira LGA, Braz HMB, de Carvalho TP, Lopes CEB, Oliveira JBS, de Paula NF, de Carvalho MPN, Alves BF, Pena HFJ, and Santos RL
- Subjects
- Animals, Animals, Zoo, Brazil epidemiology, Female, Male, Monkey Diseases epidemiology, Sapajus apella parasitology, Sapajus apella physiology, Toxoplasmosis, Animal epidemiology, Alouatta, Genotype, Monkey Diseases parasitology, Toxoplasma genetics, Toxoplasmosis, Animal parasitology
- Abstract
Background: Toxoplasmosis is a zoonotic disease that affects humans and warm-blooded animals. This study describes an outbreak of toxoplasmosis in howler monkeys (Alouatta sp.) and survival of capuchins (Sapajus apella), under the same environmental conditions., Methods: Howler monkeys were submitted to post-mortem examination. Tissue samples were processed to histopathology and immunohistochemistry to detect lesions and tachyzoites of Toxoplasma gondii. Tissue samples were also frozen and submitted to PCR and genotyping of T. gondii., Results: Typical lesions were observed in several organs including the liver, lymph node, and brain, with intralesional cysts and tachyzoites of T. gondii demonstrated by immunohistochemistry. T. gondii genomic sequences were amplified by PCR, and genotyping characterized the same T. gondii clone in all howler monkeys., Conclusions: Our results support the notion that some species of neotropical primates are highly susceptible to toxoplasmosis and the hypothesis that capuchins (S. apella) may be resistant., (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2021
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