1. Rose Bengal incorporated to α-cyclodextrin microparticles for photodynamic therapy against the cariogenic microorganism Streptococcus mutans.
- Author
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Alexandrino FJR, Bezerra EM, Da Costa RF, Cavalcante LRL, Sales FAM, Francisco TS, Rodrigues LKA, de Brito DHA, Ricardo NMPS, Costa SN, de Lima-Neto P, Barroso-Neto IL, Caetano EWS, and Freire VN
- Subjects
- Biofilms, Microscopy, Electron, Scanning, Particle Size, Photosensitizing Agents administration & dosage, Rose Bengal administration & dosage, Spectrophotometry, Infrared, Photochemotherapy methods, Photosensitizing Agents pharmacology, Rose Bengal pharmacology, Streptococcus mutans drug effects, alpha-Cyclodextrins chemistry
- Abstract
Rose Bengal@α-cyclodextrin (RB@α-CD) microparticles (μPs) were prepared and the RB inclusion in α-CD was experimentally demonstrated through infrared, UV-VIS absorption spectroscopy and cyclic voltammetry. The RB inclusion in α-CD was theoretically investigated using classical molecular mechanics calculations, the simulation results showing that RB can be included in both the narrow and wide apertures of the α-cyclodextrin ring with configurations exhibiting average binding energies of about 27 kcal mol
-1 . The prepared RB@α-CD microparticles were characterized through Scanning Electron Microscopy (SEM) and it was demonstrated that they are highly efficient in the photodynamic therapy against a Streptococcus mutans (the main bacteria of cariogenic dental plaque) suspension, as a concentration of RB@α-CD μPs 10 times smaller than the usual concentration of pure RB is still capable to produce significant antibacterial activity., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2019
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