1. Common site of mutation in the erbB gene of avian erythroblastosis virus mutants that are temperature sensitive for transformation.
- Author
-
Scotting P, Vennstrom B, Jansen M, Graf T, Beug H, and Hayman MJ
- Subjects
- Amino Acid Sequence, Base Sequence, Cell Line, Cloning, Molecular, DNA Restriction Enzymes, DNA, Viral genetics, Molecular Sequence Data, Oncogene Proteins v-erbB, Plasmids, Temperature, Alpharetrovirus genetics, Avian Leukosis Virus genetics, Cell Transformation, Neoplastic, Genes, Viral, Mutation, Oncogene Proteins, Viral, Viral Proteins genetics
- Abstract
The genome of the avian erythroblastosis virus temperature sensitive mutant ts34 was cloned from a cell line that was shown to contain a single integrated copy of the virus. The mutation was localized to the v-erbB gene by making chimeric viruses between the mutant genome and that of wildtype. Sequencing of the mutant v-erbB gene revealed a single amino acid change of a histidine to an aspartate residue at a position equivalent to amino-acid 826 of the human epidermal growth factor receptor. Interestingly this is the identical mutation to that recently reported for another temperature sensitive mutant ts167, indicating that this may be a hot spot for mutations in the v-erbB gene that give rise to ts transformation mutants. In addition, the different biological phenotypes of ts34 and ts167 are suggested to be due to an additional mutation in v-erbA in ts167.
- Published
- 1987