1. The glutaredoxin ATGRXS13 is required to facilitate Botrytis cinerea infection of Arabidopsis thaliana plants
- Author
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La Camera, Sylvain, L'Haridon, Floriane, Astier, Jérémy, Zander, Mark, Abou-Mansour, Eliane, Page, Gonzague, Thurow, Corinna, Wendehenne, David, Gatz, Christiane, Métraux, Jean-Pierre, Lamotte, Olivier, PhyMoTS, Laboratoire de catalyse en chimie organique (LACCO), Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers, Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Management et Relations internationales, ESG, Catalyse en chimie organique ( LACCO ), Université de Poitiers-Centre National de la Recherche Scientifique ( CNRS ) -Université de Poitiers-Centre National de la Recherche Scientifique ( CNRS ), Plante - microbe - environnement : biochimie, biologie cellulaire et écologie ( PMEBBCE ), Etablissement National d'Enseignement Supérieur Agronomique de Dijon ( ENESAD ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Equipe Physiologie Moléculaire du Transport de Sucres (PhyMoTS), Université de Poitiers-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)
- Subjects
MESH: Signal Transduction ,MESH : Molecular Sequence Data ,MESH : Transcription Factors ,Arabidopsis ,MESH : Alternative Splicing ,MESH: Amino Acid Sequence ,MESH: RNA, Plant ,MESH : Arabidopsis Proteins ,necrotrophic pathogen ,MESH : Arabidopsis ,MESH: Plant Diseases ,[ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology ,Gene Expression Regulation, Plant ,MESH: Arabidopsis ,Cloning, Molecular ,Disease Resistance ,Oligonucleotide Array Sequence Analysis ,MESH : Amino Acid Sequence ,MESH : Plant Diseases ,MESH: Alternative Splicing ,MESH : Oxylipins ,food and beverages ,MESH : Glutaredoxins ,MESH: Transcription Factors ,MESH : Oligonucleotide Array Sequence Analysis ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,RNA, Plant ,MESH: Salicylic Acid ,Botrytis ,botrytis cinerea ,Salicylic Acid ,Signal Transduction ,MESH : Botrytis ,MESH : Cloning, Molecular ,MESH: Oxylipins ,MESH: Glutaredoxins ,Molecular Sequence Data ,MESH: Disease Resistance ,MESH: Arabidopsis Proteins ,Cyclopentanes ,MESH: Botrytis ,MESH : Disease Resistance ,MESH: Cloning, Molecular ,Amino Acid Sequence ,Oxylipins ,MESH: Gene Expression Regulation, Plant ,Glutaredoxins ,Plant Diseases ,MESH : Signal Transduction ,MESH : Mutagenesis, Insertional ,MESH: Molecular Sequence Data ,MESH : RNA, Plant ,MESH : Salicylic Acid ,Arabidopsis Proteins ,jasmonic acid ,fungi ,glutaredoxin ,Alternative Splicing ,Mutagenesis, Insertional ,MESH: Mutagenesis, Insertional ,MESH: Oligonucleotide Array Sequence Analysis ,MESH: Cyclopentanes ,MESH : Cyclopentanes ,MESH : Gene Expression Regulation, Plant ,Transcription Factors - Abstract
International audience; Botrytis cinerea is a major pre- and post-harvest necrotrophic pathogen with a broad host range that causes substantial crop losses. The plant hormone jasmonic acid (JA) is involved in the basal resistance against this fungus. Despite basal resistance, virulent strains of B. cinerea can cause disease on Arabidopsis thaliana and virulent pathogens can interfere with the metabolism of the host in a way to facilitate infection of the plant. However, plant genes that are required by the pathogen for infection remain poorly described. To find such genes, we have compared the changes in gene expression induced in A. thaliana by JA with those induced after B. cinerea using genome-wide microarrays. We have identified genes that are repressed by JA but that are induced by B. cinerea. In this study, we describe one candidate gene, ATGRXS13, that encodes for a putative glutaredoxin and that exhibits such a crossed expression. In plants that are infected by this necrotrophic fungus, ATGRXS13 expression was negatively controlled by JA and TGA transcription factors but also through a JA-salicylic acid (SA) cross-talk mechanism as B. cinerea induced SA production that positively controlled ATGRXS13 expression. Furthermore, plants impaired in ATGRXS13 exhibited resistance to B. cinerea. Finally, we present a model whereby B. cinerea takes advantage of defence signalling pathways of the plant to help the colonization of its host.
- Published
- 2011
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