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Objective: The study proposes using the dolomite buffer capacity (BCDolomite) as a method of estimating the lime requirement in soils that agronomically require a pH increase. Design/methodology/approach: In six soil samples with different levels of response to the application of dolomite, the organic matter (OM), the content of sand, silt and clay, pH, K, Ca, Mg and the potential acidity (Al-H) were determined. Assuming an apparent density of 1000 kg/m3 and a volume per hectare of 2000 m3, the soils were placed in polyethylene containers and treated with dolomite in doses equivalent to 0, 750, 1500, 2250 and 3000 kg/ha, establishing the BCDolomite as the inverse of the slope resulting from the relationship between the pH and the dose of dolomite. Results: In soils with low response to the dolomite, the content of clay and OM was 280 and 26 g/kg, respectively, and in high response soils, the content of clay and OM was 240 and 47 g/kg, respectively. In all cases, a simple and direct linear relationship was observed (R2-0.85; p=0.05) in the relationship between pH and dose of dolomite. Limitations on study/implications: The results obtained under controlled conditions show that BCDolomite constitutes a viable method to estimate the lime requirement. Findings/conclusions: The BCDolomite showed sensitivity to the complexity of the clay fraction and to the organic matter in the soil, for which the dolomite requirement is equal to the product of the desired pH increase and the BCDolomite. [ABSTRACT FROM AUTHOR]

Rhizosphere microbes are important for plant tolerance to various soil stresses. Rice is the most aluminum (Al)-tolerant small grain cereal crop species, but the link between rice Al tolerance and rhizosphere microbiota remains unclear. This study aimed to investigate the microbial community structure of aluminum-sensitive and Al-tolerant rice varieties in acid sulfate soil under liming and non-liming conditions. We analyzed the rice biomass and mineral element contents of rice plants as well as the chemical properties and microbial (archaea, bacteria, and fungi) communities of rhizosphere and bulk soil samples. The results showed that the Al-tolerant rice genotype grew better and was able to take up more phosphorus from the acid sulfate soil than the Al-sensitive genotype. Liming was the main factor altering the microbial diversity and community structure, followed by rhizosphere effects. In the absence of liming effects, the rice genotypes shifted the community structure of bacteria and fungi, which accounted for the observed variation in the rice biomass. The Al-tolerant rice genotype recruited specific bacterial and fungal taxa (Bacillus , Pseudomonas , Aspergillus , and Rhizopus) associated with phosphorus solubilization and plant growth promotion. The soil microbial co-occurrence network of the Al-tolerant rice genotype was more complex than that of the Al-sensitive rice genotype. In conclusion, the bacterial and fungal community in the rhizosphere has genotype-dependent effects on rice Al tolerance. Aluminum-tolerant rice genotypes recruit specific microbial taxa, especially phosphorus-solubilizing microorganisms, and are associated with complex microbial co-occurrence networks, which may enhance rice growth in acid sulfate soil. [ABSTRACT FROM AUTHOR]

• Surface liming reduced soil acidity down to −60 cm one year after its application. • Liming increased soybean grain yield by 9%, but it had no effect on cereal crops. • The lime reactivity had no effect on crop grain yield. • Gypsum did not increase crop grain yield in this moderate acidic Oxisol. • Combined application of gypsum and lime did not benefit crop yields. The low solubility of lime (CaCO 3) and the absence of soil disturbance in fields under no-tillage (NT) may diminish the effectiveness of surface liming to reduce exchangeable Al3+ (toxic to plants) and increase the base saturation in deep soil layers. However, the effects of subsurface soil acidity can be attenuated by applying agricultural gypsum (CaSO 4 ⋅2H 2 O), which is more soluble than lime, and thus, can leached bases (exchangeable Ca2+, Mg2+ and K+) and S-SO 4 2− to deeper soil layers as well as decrease Al3+ toxicity to plants. Therefore, gypsum can be applied individually or in combination with lime. Herein, we conducted a field experiment aiming to evaluate short- (1 year) and long-term (11 years) effects of surface liming and gypsum application on the chemical properties of the 0.00–0.10, 0.10–0.20, 0.20–0.40 and 0.40–0.60 m soil layers and also on crop yield of 22 crop seasons (i.e., 10 soybean, 3 maize, 4 white oat, 3 wheat and 2 barley crops). The study was performed in a clayey Typic Hapludox of moderate acidity managed under NT for more than 25 years in Guarapuava, Paraná State, southern Brazil. Three soil surface-applied lime strategies were tested in order to raise base saturation to 70%, namely: (a) 4.62 Mg ha–1 rate of lime of low effective calcium carbonate equivalent (ECCE = 75%); (b) 3.47 Mg ha–1 rate of lime with a high ECCE value (101%); and (c) the same rate of high-ECCE lime split in three applications (i.e., 1/3 at the start of experiment; 1/3 after 1 year and 1/3 after 2 years). A control treatment without liming was also conducted. The liming strategies were combined with four rates of agricultural gypsum (i.e., 0, 3, 6 and 9 Mg ha–1) applied at the start of the experiment. Our findings did not reveal synergistic effect of lime and gypsum on soil chemical properties nor on crop yield. Gypsum resulted in very slight gains in grain yield (4% on average) and limited to just 25% of cereal (corn and winter cereals) crop seasons. On the other hand, liming increased soybean yields by 14% in 40% of crop seasons. Gypsum was more efficient than lime in raising exchangeable Ca2+ levels up to −0.60 m in the short term; however, lime promoted greater reduction of soil acidity and had a more marked residual effect on exchangeable Ca2+ contents than gypsum. The liming strategies did no promote substantial differences in crop yield, but low-ECCE lime sustained better soil chemical condition to plant growth for a longer period. Surface liming efficiently reduced subsurface acidity in a moderately acidic Oxisol managed under NT even in the short-term, promoting increments on soybean crop yields. In this case, the application of gypsum rates did not bring additional benefits to soil or plant yield. [ABSTRACT FROM AUTHOR]

Soils of the tropics and sub-tropics are typically acid and depleted of soluble sources of silicon (Si) due to weathering and leaching associated with high rainfall and temperatures. Together with intensive cropping, this leads to marginal or deficient plant Si levels in Si-accumulating crops such as rice and sugarcane. Although such deficiencies can be corrected with exogenous application of Si sources, there is controversy over the effectiveness of sources in relation to their total Si content, and their capacity to raise soil and plant Si concentrations. This study tested the hypothesis that the total Si content and provision of plant-available Si from six sources directly affects subsequent plant Si uptake as reflected in leaf Si concentration. Two trials with potted cane plants were established with the following Si sources as treatments: calcium silicate slag, fused magnesium (thermo) phosphate, volcanic rock dust, magnesium silicate, and granular potassium silicate. Silicon sources were applied at rates intended to achieve equivalent elemental soil Si concentrations; controls were untreated or lime-treated. Analyses were conducted to determine soil and leaf elemental concentrations. Among the sources, calcium silicate produced the highest leaf Si concentrations, yet lower plant-available soil Si concentrations than the thermophosphate. The latter, with slightly higher total Si than the slag, produced substantially greater increases in soil Si than all other products, yet did not significantly raise leaf Si above the controls. All other sources did not significantly increase soil or leaf Si concentrations, despite their high Si content. Hence, the total Si content of sources does not necessarily concur with a product's provision of soluble soil Si and subsequent plant uptake. Furthermore, even where soil pH was raised, plant uptake from thermophosphate was well below expectation, possibly due to its limited liming capacity. The ability of the calcium silicate to provide Si while simultaneously and significantly increasing soil pH, and thereby reducing reaction of Si with exchangeable Al3+, is proposed as a potential explanation for the greater Si uptake into the shoot from this source. [ABSTRACT FROM AUTHOR]

Aluminum (Al) toxicity is the key factor limiting wheat production in acid soils. Soil liming has been used widely to increase the soil pH, but due to its high cost, breeding tolerant cultivars is more cost-effective mean to mitigate the problem. Tolerant cultivars could be developed by traditional breeding, genetic transformation or introgression of genes from wild relatives. We used 30 wheat alien chromosome addition lines to identify new genetic resources to improve wheat tolerance to Al and to identify the chromosomes harboring the tolerance genes. We evaluated these lines and their wheat background Chinese Spring for Al tolerance in hydroponic culture at various Al concentrations. We also investigated Al uptake, oxidative stress and cell membrane integrity. The L. racemosus chromosomes A and E significantly enhanced the Al tolerance of the wheat in term of relative root growth. At the highest Al concentration tested (200 μM), line E had the greatest tolerance. The introgressed chromosomes did not affect Al uptake of the tolerant lines. We attribute the improved tolerance conferred by chromosome E to improved cell membrane integrity. Chromosome engineering with these two lines could produce Al-tolerant wheat cultivars. [ABSTRACT FROM AUTHOR]

Arbuscular mycorrhizal fungi (AMF) are important in plant nutrient uptake, but their function is prone to environmental constraints including soil factors that may suppress AMF transfer of phosphorus (P) from the soil to the plant. The objective of this study was to disentangle the biotic and abiotic components of AMF-suppressive soils. Suppression was measured in terms of AMF-mediated plant uptake of 33P mixed into a patch of soil and treatments included soil sterilization, soil mixing, pH manipulation and inoculation with isolated soil fungi. The degree of suppression was compared to volatile organic compound (VOC) production by isolated fungi and to multi-element analysis of soils. For a selected suppressive soil, sterilization and soil mixing experiments confirmed a biotic component of suppression. A Fusarium isolate from that soil suppressed the AMF activity and produced greater amounts than other fungal isolates of the antimicrobial VOC trichodiene (a trichothecene toxin precursor), beta-chamigrene, alpha-cuprenene and p-xylene. These metabolites deserve further attention when unravelling the chemical background behind the suppression of AMF activity by soil microorganisms. For the abiotic component of suppression, soil liming and acidification experiments confirmed that suppression was strongest at low pH. The pH effect might be associated with changed availability of specific suppressive elements. Indeed 33P uptake from the soil patches correlated negatively to Al levels and Al toxicity seems to play a major role in the AMF suppressiveness at pH below 5.0–5.2. However, the documentation of a biotic component of suppression for both low and high pH soils leads to the conclusion that biotic and abiotic components of suppression may act in parallel in some soils. The current insight into the components of soil suppressiveness of the AMF activity aids to develop management practices that allow for optimization of AMF functionality. • In some soils, suppression of AMF activity had a biotic component. • A Fusarium isolate from a suppressive soil inhibited the AMF activity. • This isolate produced greater amounts of antimicrobial VOCs than other isolates. • AMF suppression increased at low pH across several soils. • For these soils, Al toxicity played a major role in suppressiveness at pH ≤ 5.0 [ABSTRACT FROM AUTHOR]