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In this study, we monitored the effect of Al3+ ions on mesenchymal cells (KUSA-A1) and human fibroblasts (NHDF) by means of in vitro experiments by culturing the cells with addition of small concentrations of aluminum ions (i.e. , 0.1, 1, 10, and 100 ppm). Bone formation test was then conducted using KUSA-A1. Small concentrations of aluminum ions delayed but did not completely inhibit cell proliferation. The amount of bone tissue decreased as the concentration of Al3+ increased and crystallinity changes were also detected by Raman spectroscopic experiments. Moreover, Al3+ ions greatly affected both structure and chemistry of bone tissues with mineral nodules becoming larger and atomic substitution of Ca with Al in bone tissue being more preponderant with increasing Al3+ concentration. Such effects in turn impaired the balance between mineral and collagen in the formed bone tissue. [Display omitted] • The effect for cell proliferation of aluminum ions depends on cell types. • KUSA-A1's proliferation was promoted with increasing the amount of aluminum ions. • Aluminum substitutes calcium in the mineral apatite of bone tissues. • Larger bone mineral nodules were observed. [ABSTRACT FROM AUTHOR]