1. A new coumarin-based fluorescence "turn-on" sensor for Al(III) ions and its bioimaging in cell.
- Author
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Jin, Lei, Wang, Wenling, Shen, Zheyu, Xu, Jiahao, Wang, Qingming, and Zhao, Chengying
- Subjects
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CELL imaging , *AQUEOUS solutions , *DETECTION limit , *IONS , *FLUORESCENCE , *DETECTORS - Abstract
A new coumarin-based probe 3-acetyl-7-diethylaminocoumarinthiosemicarbazone (DCT) is synthesized for Al3+ fluorescence detection in CH 3 CH 2 OH/H 2 O (6 : 4, v / v). The fluorescence intensity at 428 nm was enhanced about 15-fold with the fluorescence changed from colorless to blue. The binding constantof Al3+ with DCT is 5.04 × 106 M−1. The limit of detection (LOD) of Al3+ is calculated to be 82 nM. Furthermore, the binding details are determined by Job's plot and ESI - MS studies, which indicated that a stoichiometric ratio of 1 : 1 complex is formed between Al3+ and DCT. Cell imaging confirmed that DCT could useful for detecting Al3+ in HepG2 cells. • Probe DCT is highly selective and sensitive towards Al3+in aqueous solution phase with LOD is 82 nM,which could monitor intracellular Al3+ levels. • The mechanism indicated that a ratio of 1 : 1 complex is formed between Al3+ and DCT. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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