1. Vertical Bone Augmentation Using Deproteinized Bovine Bone Mineral, Absorbable Collagen Sponge, and Recombinant Human Bone Morphogenetic Protein-2: An In Vivo Study in Rabbits
- Author
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Yeon Jung Kim, Fausto Rioiti Horiguti, Joni Augusto Cirelli, Guilherme Piragine Contador, Rafael Scaf de Molon, Vinicius Ibiapina Mascarenhas, Ana Paula de Souza Faloni, Wilson Roberto Sendyk, and Marco Antonio Coelho
- Subjects
Vascular Endothelial Growth Factor A ,Pathology ,medicine.medical_specialty ,X-ray microtomography ,Bone Regeneration ,Bone density ,0206 medical engineering ,Bone Morphogenetic Protein 2 ,02 engineering and technology ,Bone morphogenetic protein ,Bone morphogenetic protein 2 ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,Bone Density ,Transforming Growth Factor beta ,Absorbable Implants ,medicine ,Animals ,Tibia ,Bone mineral ,Biological Products ,Minerals ,Bone Transplantation ,Chemistry ,030206 dentistry ,General Medicine ,Alveolar Ridge Augmentation ,X-Ray Microtomography ,020601 biomedical engineering ,Recombinant Proteins ,Disease Models, Animal ,Cattle ,Female ,Collagen ,Rabbits ,Oral Surgery - Abstract
Purpose The objective of this investigation was to assess vertical bone augmentation using deproteinized bovine bone mineral (DBBM) infused or not with recombinant human bone morphogenetic protein (rhBMP-2) in rabbit tibiae. Materials and methods A total of 18 female rabbits (New Zealand) received two blocks of DBBM in each tibia. The DBBM blocks were randomly assigned into four experimental groups: DBBM (only the bone graft); DBBM associated with absorbable collagen sponge (ACS); DBBM plus rhBMP-2 (1.5 mg/mL); and DBBM infused with rhBMP-2 (1.5 mg/mL) in an ACS carrier. Animals were sacrificed after 12 weeks, and the tibiae containing the DBBM blocks were dissected and analyzed radiographically (microcomputed tomography [micro-CT]), histologically, and immunohistochemically. Results Micro-CT analysis showed a considerable increase in bone volume (BV) and BV/tissue volume in the rhBMP-2/ACS group compared with all the others. Trabeculae thickness also increased in the rhBMP-2/ACS group compared with the DBBM/ACS group. Trabecular number, separation, and bone mineral density were not different among groups. Histomorphometric evaluation showed increased newly formed bone in the rhBMP-2/ACS group compared with the DBBM and DBBM/ACS groups. The amount of residual bone graft was statistically higher in the rhBMP-2 groups compared with the DBBM/ACS group. Immunohistochemical analysis showed that the vascular endothelial growth factor (VEGF) expression was more intense in the rhBMP-2/ACS group compared with the DBBM/ACS group. The immunopositivity for type 1 collagen tended to be higher in the two groups with rhBMP-2. Conclusion Collectively, the results of this study suggest that the addition of rhBMP-2 in an ACS carrier placed on top of the DBBM graft enhanced bone formation in this animal model.
- Published
- 2018