1. Molecular cloning and nucleotide sequence of cDNA encoding a functional murine low-density-lipoprotein receptor
- Author
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Dichek Da, Anderson Wf, Polvino Wj, and Mason J
- Subjects
Genetic Vectors ,Molecular Sequence Data ,Molecular cloning ,Biology ,Mice ,chemistry.chemical_compound ,Complementary DNA ,Genetics ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Receptor ,Peptide sequence ,Expression vector ,Base Sequence ,Sequence Homology, Amino Acid ,Cholesterol ,Nucleic acid sequence ,DNA ,Cell Biology ,General Medicine ,Molecular biology ,Introns ,Recombinant Proteins ,Lipoproteins, LDL ,Retroviridae ,Genes ,Receptors, LDL ,chemistry ,Biochemistry ,LDL receptor ,lipids (amino acids, peptides, and proteins) - Abstract
The low-density-lipoprotein (LDL) receptor is an important mediator of mammalian cholesterol metabolism; its congenital absence in humans is characterized by hypercholesterolemia, atherosclerosis, and coronary artery disease. We report here the identification and cloning of a cDNA encoding the murine LDL receptor. The cDNA insert is 4467 base pairs in length and the deduced amino acid sequence bears 78.2% homology with the reported human sequence. This murine cDNA was subcloned into a retroviral-based expression vector, LmLSN1, and transfected into 3T3 cells. The production of functional LDL receptor was confirmed by ligand binding of DiI-LDL cholesterol.
- Published
- 1992
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