1. Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications.
- Author
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Brumbaugh K, Liao WC, Houchins JP, Cooper J, and Stoesz S
- Subjects
- Amino Acids metabolism, Animals, Antibody Formation, Blotting, Western, Cell Surface Display Techniques, Drug Discovery methods, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Gene Expression Profiling, Humans, Hybridomas immunology, Hybridomas metabolism, Immunohistochemistry, Mice, Phosphoproteins genetics, Phosphoproteins metabolism, Phosphorylation, Protein Array Analysis methods, Reproducibility of Results, Amino Acids immunology, Antibodies immunology, Antibody Specificity immunology, Phosphoproteins immunology
- Abstract
Phosphate addition is a posttranslational modification of proteins, and this modification can affect the activity and other properties of intracellular proteins. Different animal species can be used to generate phosphosite-specific antibodies as either polyclonals or monoclonals, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is vital for their use in proteomics and profiling of disease.
- Published
- 2017
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