1. An Orbitide from Ratibida columnifera Seed Containing 16 Amino Acid Residues.
- Author
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Fisher MF, Payne CD, Rosengren KJ, and Mylne JS
- Subjects
- Amino Acid Sequence, Mass Spectrometry, Nuclear Magnetic Resonance, Biomolecular, Peptides, Cyclic chemistry, Protein Conformation, Amino Acids analysis, Peptides, Cyclic isolation & purification, Ratibida embryology, Seeds chemistry
- Abstract
Cyclic peptides are abundant in plants and have attracted interest due to their bioactivity and potential as drug scaffolds. Orbitides are head-to-tail cyclic peptides that are ribosomally synthesized, post-translationally modified, and lack disulfide bonds. All known orbitides contain 5-12 amino acid residues. Here we describe PLP-53, a novel orbitide from the seed of Ratibida columnifera . PLP-53 consists of 16 amino acids, four residues larger than any known orbitide. NMR structural studies showed that, compared to previously characterized orbitides, PLP-53 is more flexible and, under the studied conditions, did not adopt a single ordered conformation based on analysis of NOEs and chemical shifts.
- Published
- 2019
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