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Your search keyword '"Krogager, Toke P."' showing total 3 results
Start Over Author "Krogager, Toke P." Topic amino acyl-trna synthetases
3 results on '"Krogager, Toke P."'

1. Labeling and identifying cell-specific proteomes in the mouse brain.

Author

Krogager TP, Ernst RJ, Elliott TS, Calo L, Beránek V, Ciabatti E, Spillantini MG, Tripodi M, Hastings MH, and Chin JW

Subjects
Amino Acids, Animals, Gene Expression Regulation, Enzymologic genetics, Mice, Neuroglia metabolism, RNA, Transfer genetics, Amino Acyl-tRNA Synthetases genetics, Brain metabolism, Neurons metabolism, Proteome genetics
Abstract

We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.

Published
2018
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2. Genetic code expansion in the mouse brain.

Author

Ernst RJ, Krogager TP, Maywood ES, Zanchi R, Beránek V, Elliott TS, Barry NP, Hastings MH, and Chin JW

Subjects
Amino Acids metabolism, Animals, Dependovirus genetics, Methanosarcina genetics, Mice, Molecular Structure, RNA, Transfer metabolism, Amino Acids chemistry, Amino Acids genetics, Amino Acyl-tRNA Synthetases metabolism, Brain cytology, Brain metabolism, Genetic Code genetics, RNA, Transfer genetics
Abstract

Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice., Competing Interests: The authors declare no competing financial interests.

Published
2016
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3. Labeling and identifying cell-specific proteomes in the mouse brain

Author

Krogager, Toke P, Ernst, Russell J, Elliott, Thomas S, Calo, Laura, Beránek, Václav, Ciabatti, Ernesto, Spillantini, Maria Grazia, Tripodi, Marco, Hastings, Michael H, and Chin, Jason W

Subjects
Amino Acyl-tRNA Synthetases, Neurons, Mice, Proteome, RNA, Transfer, Animals, Brain, Amino Acids, Neuroglia, Gene Expression Regulation, Enzymologic, 3. Good health
Abstract

We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain., Michael J Fox Foundation (MJFF) (unknown), Parkinson's UK (None), Cure Parkinson's Trust (unknown)

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