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This study investigated the performance of thermophilic–mesophilic (T-M) and mesophilic–thermophilic (M-T) two-phase sludge anaerobic digestion at different inoculation proportions after a change in digestion temperature. After temperature change, the pH, total ammonia nitrogen (TAN), free ammonia nitrogen (FAN), solubility chemical oxygen demand (SCOD), and total alkalinity (TA) levels of two-phase digesters were between thermophilic control digesters and mesophilic control digesters. However, the volatile fatty acid (VFA) levels of two-phase digesters were higher than those of thermophilic or mesophilic control digesters. The bacteria communities of M-T two-phase digesters were more diverse than those of T-M. After a change in digestion temperature, the bacterial community was dominated by Coprothermobacter. After a change of digestion temperature, the relative abundance (RA) of Methanobacterium, Methanosaeta, and Methanospirillum of M-T two-phase digesters was higher than that of T-M two-phase digesters. In comparison, the RA of Methanosarcina of T-M two-phase digesters was higher than that of M-T two-phase digesters. The ultimate methane yields of thermophilic control digesters were greater than those of mesophilic control digesters. Nevertheless, the ultimate methane yield levels of M-T two-phase digesters were greater than those of T-M two-phase digesters. The ultimate methane yields of all two-phase digesters presented an earlier increase and later decrease trend with the increasing inoculation proportion. Optimal methane production condition was achieved when 15% of sludge (T-M15) was inoculated under mesophilic–thermophilic conditions, which promoted 123.6% (based on mesophilic control) or 27.4% (based on thermophilic control). An optimal inoculation proportion (about 15%) balanced the number and activity of methanogens of high-solid sludge anaerobic digestion. [ABSTRACT FROM AUTHOR]

[Display omitted] • Co-digestion improved methane yield 31.3%-68.0% at thermophilic temperature. • Co-digestion improved methane yield 83.0%-136.5% at mesophilic temperature. • The highest methane yield of anaerobic co-digestion obtained at P/S = 2:1. • NCST and TS of sediment increased with increasing initial TS of substrate. • NCST has a strong negative correlation with the water removal efficiency. The objective of this study is to assess the effects of the mixing ratio on the methane production and digestate dewaterability of co-digestion of pig manure (P) and sludge (S). Batch experiments were carried out at five different P/S mixing ratios at mesophilic and thermophilic temperatures. Compared to sludge anaerobic digestion, co-digestion of pig manure with sludge increased methane yield 83.0%-136.5% at mesophilic temperature and 31.3%-68.0% at thermophilic temperature. The normalized capillary suction time (NCST) and total solids (TS) of sediment (centrifugal dewatering) increased when pig manure proportion of substrate increased. The NCST at thermophilic temperatures (4.87–17.58 s g−1-TSS) was higher than that at mesophilic temperatures (1.89–10.95 s g−1-TSS). However, the TS of sediment was close at thermophilic and mesophilic temperatures. The results indicated that anaerobic co-digestion of pig manure and sludge at a proper mixing ratio (P/S = 2:1) provides a good choice for energy recovery and land utilization. [ABSTRACT FROM AUTHOR]