1. Thymus cDNA library survey uncovers novel features of immune molecules in Chinese giant salamander Andrias davidianus.
- Author
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Zhu R, Chen ZY, Wang J, Yuan JD, Liao XY, Gui JF, and Zhang QY
- Subjects
- Amino Acid Sequence, Amphibian Proteins metabolism, Animals, Cells, Cultured, Chemokines genetics, Chemokines metabolism, Gene Library, Immunoglobulins metabolism, Molecular Sequence Annotation, Molecular Sequence Data, Phylogeny, Protein Isoforms genetics, Protein Isoforms metabolism, Protein Transport, RNA Splicing, Ranavirus immunology, Receptors, Antigen, T-Cell, alpha-beta genetics, Receptors, Antigen, T-Cell, alpha-beta metabolism, Transcriptome, Urodela genetics, Amphibian Proteins genetics, Immunoglobulins genetics, Thymus Gland immunology, Urodela immunology
- Abstract
A ranavirus-induced thymus cDNA library was constructed from Chinese giant salamander, the largest extant amphibian species. Among the 137 putative immune-related genes derived from this library, these molecules received particular focus: immunoglobulin heavy chains (IgM, IgD, and IgY), IFN-inducible protein 6 (IFI6), and T cell receptor beta chain (TCRβ). Several unusual features were uncovered: IgD displays a structure pattern distinct from those described for other amphibians by having only four constant domains plus a hinge region. A unique IgY form (IgY(ΔFc)), previously undescribed in amphibians, is present in serum. Alternative splicing is observed to generate IgH diversification. IFI6 is newly-identified in amphibians, which occurs in two forms divergent in subcelluar distribution and antiviral activity. TCRβ immunoscope profile follows the typical vertebrate pattern, implying a polyclonal T cell repertoire. Collectively, the pioneering survey of ranavirus-induced thymus cDNA library from Chinese giant salamander reveals immune components and characteristics in this primitive amphibian., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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