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Aim: To study the microbial adhesion on different orthodontic brackets (conventional, ceramic, and self-ligating brackets). Materials and Methods: Three types of bracket systems i.e. self-ligating, conventional, and ceramic brackets were used consisting of 10 patients for each bracket system. Out of 30 patients 20 patients will be treated with conventional and ceramic brackets, in which, in one-half of the mouth steel ligature ties are placed and in the other half elastomeric rings would be placed. We collected swabs from the central incisors and first premolars of the both the right and left sides of both the maxillary and mandibular arches. The samples were collected three times from the above-mentioned teeth once prior to the placement of the brackets, the second and third samples after one and three months respectively. Result: Significant variations were between the pretreatment and after one and three months of bracket placement in all three groups. Significant increase in the microbial adhesion of aerobic and anaerobic bacteria in conventional bracket form pretreatment to one and three months after bracket placement is seen. Although the colony formed by anaerobic bacteria is more in number in comparison to the aerobic bacteria. Conclusion: Our study reveals that the most hygienic bracket is a self-ligating bracket that should be used in patients who have poor oral hygiene. We also found that using steel ligature is more suitable as compared to elastomeric ligature in both conventional and ceramic brackets. [ABSTRACT FROM AUTHOR]