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Start Over Author "Hansen, Martin C." Topic anaerobic bacteria -- physiological aspects
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1. Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration

Author

Hansen, Martin C., Palmer, Robert J., Jr., Udsen, Camilla, White, David C., and Molin, Soren

Subjects
Microbiological research -- Reports, Cytochemistry -- Research, Fluorescence -- Physiological aspects, Streptococcus -- Physiological aspects, Oxygen -- Physiological aspects, Hydrogen-ion concentration -- Physiological aspects, Anaerobic bacteria -- Physiological aspects, Gram-positive bacteria -- Research, Microbial mats -- Research, Bacterial growth -- Research, Biological sciences
Abstract

Green fluorescent protein (GFP) fluorescence in Streptococcus gordonii was assessed under low-oxygen concentration and low-pH conditions. GFP, it was found, can be used in studies of oxygen-tolerant anaerobic bacteria. Densely packed, flow cell-grown biofilms of S. gordonii do not develop oxygen gradients that inhibit GFP fluorescence development. The transient nature of GFP fluorescence in acid-producing bacteria can often be overcome in flowcells.

Published
2001

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